2007年3月
Protection induced in BALB/c mice by the high-molecular-mass (hMM) fraction of Paracoccidioides brasiliensis
MYCOPATHOLOGIA
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- 巻
- 163
- 号
- 3
- 開始ページ
- 117
- 終了ページ
- 128
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1007/s11046-007-0095-4
- 出版者・発行元
- SPRINGER
Paracoccidioidomycosis (PCM) is a granulomatous disease caused by a dimorphic fungus, Paracoccidioides brasiliensis. The present study investigated the protective activity of the P. brasiliensis high-molecular-mass (hMM) fraction (similar to 380 kDa) in experimental murine PCM. In the first step, lymphocyte proliferation and production of IFN gamma (but not IL-4) were observed in "in vitro" spleen cells (from female BALB/c mice infected (i.v.) with P. brasiliensis) that were stimulated with hMM fractions. In the second step, female BALB/c mice were previously immunized (s.c.) with hMM fraction (25 mu g/protein = F-25 and 50 mu g/protein = F-50), and the colony-forming units (CFU) of the lung and spleen, the histopathological characteristics of the granulomatous lesions, and plasmatic gp43 soluble antigens and anti-hMM IgG levels were analyzed at 28 and 56 days after infection. The lung and liver CFU were lower in mice previously immunized with the hMM fraction (P < 0.05). The granulomatous lesions revealed a greater degree of compaction and organization, with no dissemination of the fungus to other organs. Lower soluble antigen levels (P < 0.05) and higher IgG anti-hMM fraction (P < 0.05) were observed in immunized groups. The results for CFU, histopathology and antigenemia suggest that the hMM fraction has a protective effect in experimental paracoccidioidomycosis in BALB/c mice.
- リンク情報
- ID情報
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- DOI : 10.1007/s11046-007-0095-4
- ISSN : 0301-486X
- PubMed ID : 17356792
- Web of Science ID : WOS:000245134300001