2016年9月
Inhibitory Effects of Vegetable Juices on CYP3A4 Activity in Recombinant CYP3A4 and LS180 Cells
BIOLOGICAL & PHARMACEUTICAL BULLETIN
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- 巻
- 39
- 号
- 9
- 開始ページ
- 1482
- 終了ページ
- 1487
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1248/bpb.b16-00263
- 出版者・発行元
- PHARMACEUTICAL SOC JAPAN
It is thought that eating habits induces individual variation in intestinal absorption and metabolism of drugs. The objective of this research was to clarify the influence of vegetables juices on CYP3A4 activity, which is an important enzyme in intestine. Five vegetables juices (VJ-o, Kagome Original (R); VJ-g, Kagome 30 kinds of vegetables and fruits (R); VJ-p, Kagome Purple vegetables (R); VJ-r, Kagome Sweet Tomato (R); and VJ-y, Kagome Fruity Salada (R); KAGOME Co., Ltd., Aichi, Japan) were centrifuged (1630xg, 10min) and filtered using filter paper and 0.45-mu m membrane filters. In this study, recombinant CYP3A4 and L5180 cells were used for the evaluation of CYP3A4 activity. The metabolisms to 6 beta-hydroxytestosterone by recombinant CYP3A4 were significantly inhibited by VJ-o, VJ-g, and VJ-y in a preincubation time-dependent manner, and CYP3A4 activity in L5180 cells were significantly inhibited by VJ-o and VJ-y. These results show that the difference in ingestion volume of vegetable juices and vegetables might partially induce individual difference in intestinal drug metabolism.
- リンク情報
- ID情報
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- DOI : 10.1248/bpb.b16-00263
- ISSN : 0918-6158
- PubMed ID : 27582329
- Web of Science ID : WOS:000382352500012