2016年10月
Carboxyspermidine decarboxylase of the prominent intestinal microbiota species Bacteroides thetaiotaomicron is required for spermidine biosynthesis and contributes to normal growth
AMINO ACIDS
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- 巻
- 48
- 号
- 10
- 開始ページ
- 2443
- 終了ページ
- 2451
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1007/s00726-016-2233-0
- 出版者・発行元
- SPRINGER WIEN
Recent studies have indicated that polyamines produced by gut microbes significantly influence host health; however, little is known about the microbial polyamine biosynthetic pathway except for that in Escherichia coli, a minor component of the gastrointestinal microbiota. Here, we investigated the polyamine biosynthetic ability of Bacteroides thetaiotaomicron, a predominant gastrointestinal bacterial species in humans. High-performance liquid chromatography analysis revealed that B. thetaiotaomicron cultured in polyamine-free minimal medium accumulated spermidine intracellularly at least during the mid-log and stationary phases. Deletion of the gene encoding a putative carboxyspermidine decarboxylase (casdc), which converts carboxyspermidine to spermidine, resulted in the depletion of spermidine and loss of decarboxylase activity in B. thetaiotaomicron. The Delta casdc strain also showed growth defects in polyamine-free growth medium. The complemented Delta casdc strain restored the spermidine biosynthetic ability, decarboxylase activity, and growth. These results indicate that carboxyspermidine decarboxylase is essential for synthesizing spermidine in B. thetaiotaomicron and contributes to the growth of this species.
- リンク情報
- ID情報
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- DOI : 10.1007/s00726-016-2233-0
- ISSN : 0939-4451
- eISSN : 1438-2199
- PubMed ID : 27118128
- Web of Science ID : WOS:000385414500016