Sulfur and strontium isotopes (delta S-34 and Sr-87/Sr-86) were determined in 39 river water samples collected over three different cultivation periods (April, May, and June), and in several materials used for comparison (fertilizers, detergents, soils, irrigation and agricultural waters), to evaluate the impact of fertilizers on a small agricultural watershed of Lake Biwa, in central Japan. delta S-34 values in river water decreased (from +5.8 to -2.0 parts per thousand) with increasing SO4 concentrations (3.8 to 93.2 ppm) from upstream to downstream of the watershed. Comparison of river water S isotopes with those of possible source materials indicates that the enrichment of SO4 can be attributed to the dissolution of two kinds of fertilizers: (1) compound fertilizers commonly used in this area and (2) ammonium sulfate which is applied on a small scale. In contrast, Sr-87/Sr-86 values of river water decreased with time from April (avg. 0.71163), through May (avg. 0.71139), to June (avg. 0.71127). The tendency of the sample plots on the Sr-87/Sr-86 vs. I/Sr diagram suggests a time-dependent increase in the contribution of soil water to the river, which is partly affected by the Sr-bearing fertilizers. It is suggested that a maximum of 25% of dissolved Sr is derived from these fertilizers, while more than 75% of it is of rock origin. Mass balance calculations permitted us to evaluate the proportion of fertilizer contribution in each river. Combined use of S and Sr isotopes together with concentration data could be a new environmental diagnosis technique for rivers and soils in localized watersheds. (C) 2007 Elsevier B.V. All rights reserved.