2020年12月1日
Metagenomic analysis of bacterial species in tongue microbiome of current and never smokers
npj Biofilms and Microbiomes
- 巻
- 6
- 号
- 1
- 開始ページ
- 11
- 終了ページ
- 11
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1038/s41522-020-0121-6
- 出版者・発行元
- NATURE RESEARCH
Cigarette smoking affects the oral microbiome, which is related to various systemic diseases. While studies that investigated the relationship between smoking and the oral microbiome by 16S rRNA amplicon sequencing have been performed, investigations involving metagenomic sequences are rare. We investigated the bacterial species composition in the tongue microbiome, as well as single-nucleotide variant (SNV) profiles and gene content of these species, in never and current smokers by utilizing metagenomic sequences. Among 234 never smokers and 52 current smokers, beta diversity, as assessed by weighted UniFrac measure, differed between never and current smokers (pseudo-F = 8.44, R2 = 0.028, p = 0.001). Among the 26 species that had sufficient coverage, the SNV profiles of Actinomyces graevenitzii, Megasphaera micronuciformis, Rothia mucilaginosa, Veillonella dispar, and one Veillonella sp. were significantly different between never and current smokers. Analysis of gene and pathway content revealed that genes related to the lipopolysaccharide biosynthesis pathway in Veillonella dispar were present more frequently in current smokers. We found that species-level tongue microbiome differed between never and current smokers, and 5 species from never and current smokers likely harbor different strains, as suggested by the difference in SNV frequency.
- リンク情報
-
- DOI
- https://doi.org/10.1038/s41522-020-0121-6
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/32170059
- PubMed Central
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7069950
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000520976600001&DestApp=WOS_CPL
- Scopus
- https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85081973272&origin=inward 本文へのリンクあり
- Scopus Citedby
- https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85081973272&origin=inward
- URL
- http://www.nature.com/articles/s41522-020-0121-6.pdf
- URL
- http://www.nature.com/articles/s41522-020-0121-6
- ID情報
-
- DOI : 10.1038/s41522-020-0121-6
- eISSN : 2055-5008
- PubMed ID : 32170059
- PubMed Central 記事ID : PMC7069950
- SCOPUS ID : 85081973272
- Web of Science ID : WOS:000520976600001