1998年9月
The S-layer protein fromCampylobacter rectus: sequence determination and function of the recombinant protein
FEMS Microbiology Letters
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- 巻
- 166
- 号
- 2
- 開始ページ
- 275
- 終了ページ
- 281
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1111/j.1574-6968.1998.tb13901.x
- 出版者・発行元
- Oxford University Press (OUP)
The gene encoding the crystalline surface layer (S-layer) protein from Campylobacter rectus, designated sip, was sequenced and the recombinant gene product was expressed in Escherichia coli. The gene consisted of 4086 nucleotides encoding a protein with 1361 amino acids. The N-terminal amino acid sequence revealed that Sip did not contain a signal sequence, but that the initial methionine residue was processed. The deduced amino acid sequence displayed some common characteristic features of S-layer proteins previously reported. A homology search showed a high similarity to the Campylobacter fetus S-layer proteins, especially in their N-terminus. The C-terminal third of Sip exhibited homology with the RTX toxins from Gram-negative bacteria via the region including the glycine-rich repeats. The Sip protein had the same N-terminal sequence as a 104-kDa cytotoxin isolated from the culture supernatants of C. rectus. However, neither native nor recombinant Sip showed cytotoxicity against HL-60 cells or human peripheral white blood cells. These data support the idea that the N-terminus acts as an anchor to the cell surface components and that the C-terminus is involved in the assembly and/or transport of the protein. (C) 1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
- リンク情報
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- DOI
- https://doi.org/10.1111/j.1574-6968.1998.tb13901.x
- CiNii Articles
- http://ci.nii.ac.jp/naid/80010560022
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/9770285
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000076091500016&DestApp=WOS_CPL
- URL
- http://academic.oup.com/femsle/article-pdf/166/2/275/19102841/166-2-275.pdf
- ID情報
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- DOI : 10.1111/j.1574-6968.1998.tb13901.x
- ISSN : 0378-1097
- eISSN : 1574-6968
- CiNii Articles ID : 80010560022
- ORCIDのPut Code : 47542660
- PubMed ID : 9770285
- Web of Science ID : WOS:000076091500016