2004年12月
Efflux of sphingoid bases by P-glycoprotein in human intestinal Caco-2 cells
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
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- 巻
- 68
- 号
- 12
- 開始ページ
- 2541
- 終了ページ
- 2546
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1271/bbb.68.2541
- 出版者・発行元
- TAYLOR & FRANCIS LTD
The aim of this study was to determine whether sphingoid bases that originated from various dietary sources, such as mammals, plants, and fungi, are substrates for P-glycoprotein in differentiated Caco-2 cells, which are used as a model of intestinal epithelial cells. In Caco-2 cells, the uptake of sphingosine, the most common sphingoid base found in mammals, was significantly higher at physiological temperatures than those of cis/trans-8-sphingenine, trans-4, cisitrans-8-sphingadienine, 9-methyl-trans-4, trans-8-sphingadienine, or sphinganine. Verapamil, a potent P-glycoprotein inhibitor, increased the cellular accumulation of sphingoid bases, except for sphingosine, in a dose-dependent manner. Incubation with 1 mum digoxin for 48 h caused up-regulation of multidrug-resistance (MDR)1 mRNA and decreased the accumulation of sphingoid bases in Caco-2 cells, except for sphingosine. Thus P-glycoprotein probably contributes to the selective absorption of sphingosine from dietary sphingolipids in the digestive tract.
- リンク情報
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- DOI
- https://doi.org/10.1271/bbb.68.2541
- CiNii Articles
- http://ci.nii.ac.jp/naid/130000030884
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000226250500016&DestApp=WOS_CPL
- URL
- http://www.scopus.com/inward/record.url?eid=2-s2.0-13544251603&partnerID=MN8TOARS
- URL
- http://orcid.org/0000-0002-1203-5521
- ID情報
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- DOI : 10.1271/bbb.68.2541
- ISSN : 0916-8451
- eISSN : 1347-6947
- CiNii Articles ID : 130000030884
- ORCIDのPut Code : 39432153
- SCOPUS ID : 13544251603
- Web of Science ID : WOS:000226250500016