Multi-layered hybrid films comprised of amphiphilic cationic iridium(III) complexes and the exfoliated layers of synthetic saponite were prepared by the modified Langmuir-Blodgett method. Two iridium(III) complexes, [Ir(dfppy)(2)(dc9bpy)](+) (dfppyH = 2-(4',6'-difluorophenyl)pyridine; dc9bpy = 4,4'-dinonyl-2,2'-bipyridine) and [Ir(dfppy)(2)(dc9bpy)(+) (piqH = 1-phenyisoquinoline), were used as blue (donor) and red emitters (acceptor), respectively. A non-luminescent hybrid layer of stearylammonium and synthetic saponite was deposited in a layer-by-layer manner to vary the distance between the donor and acceptor layers. Energy transfer was analysed according to the Forster-type mechanism with a focus on the dependence of its efficiency on the interlayer distance.