SAKUMA TETSUSHI

J-GLOBAL         Last updated: Sep 6, 2019 at 02:59
 
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Name
SAKUMA TETSUSHI
Nickname
Tetsushi Sakuma
E-mail
tetsushi-sakumahiroshima-u.ac.jp
Affiliation
Hiroshima University
Section
.
Job title
講師
Degree
Doctor of Science (Ph.D.)(Hiroshima University), Master of Science(Hiroshima University)
Research funding number
90711143
ORCID ID
0000-0003-0396-1563

Profile

I am investigating the genome editing systems, methods, and techniques, and applying them in various cells and organisms with my great collaborators.

Research Areas

 
 

Academic & Professional Experience

 
Apr 2012
 - 
Mar 2013
JSPS Research Fellow (PD), Japan Society for the Promotion of Science (JSPS)
 
Apr 2013
 - 
Mar 2015
Assistant Professor (Special Appointment), Graduate School of Science, Hiroshima University
 
Apr 2015
 - 
Mar 2018
Lecturer (Special Appointment), Graduate School of Science, Hiroshima University
 
Apr 2018
 - 
Mar 2019
Lecturer, Graduate School of Science, Hiroshima University
 
Apr 2019
   
 
Lecturer, Graduate School of Integrated Sciences for Life, Hiroshima University
 

Education

 
Apr 2002
 - 
Mar 2006
Osaka University
 
Apr 2006
 - 
Mar 2008
Departmet of Biological Science, Faculty of Science, Hiroshima University
 
Apr 2008
 - 
Mar 2010
Department of Biological Science, Graduate School of Science, Hiroshima University
 
Apr 2010
 - 
Mar 2012
Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University
 

Published Papers

 
Watanabe, Masahito; Nakano, Kazuaki; Uchikura, Ayuko; Matsunari, Hitomi; Yashima, Sayaka; Umeyama, Kazuhiro; Takayanagi, Shuko; Sakuma, Tetsushi; Yamamoto, Takashi; Morita, Sumiyo; Horii, Takuro; Hatada, Izuho; Nishinakamura, Ryuichi; Nakauchi, Hiromitsu; Nagashima, Hiroshi
SCIENTIFIC REPORTS   9    May 2019   [Refereed]
To combat organ shortage in transplantation medicine, a novel strategy has been proposed to generate human organs from exogenous pluripotent stem cells utilizing the developmental mechanisms of pig embryos/foetuses. Genetically modified pigs missi...
Kohara, Hiroshi; Utsugisawa, Taiju; Sakamoto, Chika; Hirose, Lisa; Ogawa, Yoshie; Ogura, Hiromi; Sugawara, Ai; Liao, Jiyuan; Aoki, Takako; Iwasaki, Takuya; Asai, Takayoshi; Doisaki, Sayoko; Okuno, Yusuke; Muramatsu, Hideki; Abe, Takaaki; Kurita, Ryo; Miyamoto, Shohei; Sakuma, Tetsushi; Shiba, Masayuki; Yamamoto, Takashi; Ohga, Shouichi; Yoshida, Kenichi; Ogawa, Seishi; Ito, Etsuro; Kojima, Seiji; Kanno, Hitoshi; Tani, Kenzaburo
EXPERIMENTAL HEMATOLOGY   73 25-37   May 2019   [Refereed]
Kruppel-like factor 1 (KLF1), a transcription factor controlling definitive erythropoiesis, is involved in sequential control of terminal cell division and enucleation via fine regulation of key cell cycle regulator gene expression in erythroid li...
Tsuda, Masataka; Ogawa, Saki; Ooka, Masato; Kobayashi, Kaori; Hirota, Kouji; Wakasugi, Mitsuo; Matsunaga, Tsukasa; Sakuma, Tetsushi; Yamamoto, Takashi; Chikuma, Shunsuke; Sasanuma, Hiroyuki; Debatisse, Michelle; Doherty, Aidan J.; Fuchs, Robert P.; Takeda, Shunichi
PLOS ONE   14(3)    Mar 2019   [Refereed]
Replicative DNA polymerases are frequently stalled at damaged template strands. Stalled replication forks are restored by the DNA damage tolerance (DDT) pathways, error-prone translesion DNA synthesis (TLS) to cope with excessive DNA damage, and e...
Nishitani, Ai; Kunisawa, Naofumi; Sugimura, Taketoshi; Sato, Kazuaki; Yoshida, Yusaku; Suzuki, Toshiro; Sakuma, Tetsushi; Yamamoto, Takashi; Asano, Masahide; Saito, Yasuhiko; Ohno, Yukihiro; Kuramoto, Takashi
BRAIN RESEARCH   1706 209-217   Mar 2019   [Refereed]
Hyperpolarized-activated cyclic nucleotide-gated (HCN) channels underlie hyperpolarization-activated current (I-h) and are involved in controlling the excitability and electrical responsiveness of neurons. Absence epilepsy is clinically defined by...
Kazuki, Yasuhiro; Kobayashi, Kaoru; Hirabayashi, Masumi; Abe, Satoshi; Kajitani, Naoyo; Kazuki, Kanako; Takehara, Shoko; Takiguchi, Masato; Satoh, Daisuke; Kuze, Jiro; Sakuma, Tetsushi; Kaneko, Takehito; Mashimo, Tomoji; Osamura, Minori; Hashimoto, Mari; Wakatsuki, Riko; Hirashima, Rika; Fujiwara, Ryoichi; Deguchi, Tsuneo; Kurihara, Atsushi; Tsukazaki, Yasuko; Senda, Naoto; Yamamoto, Takashi; Scheer, Nico; Oshimura, Mitsuo
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   116(8) 3072-3081   Feb 2019   [Refereed]
Although "genomically" humanized animals are invaluable tools for generating human disease models as well as for biomedical research, their development has been mainly restricted to mice via established transgenic-based and embryonic stem cell-bas...
Harata, Ayano; Hirakawa, Mika; Sakuma, Tetsushi; Yamamoto, Takashi; Hashimoto, Chikara
DEVELOPMENT GROWTH & DIFFERENTIATION   61(2) 186-197   Feb 2019   [Refereed]
Vertebrates have unique head structures that are mainly composed of the central nervous system, the neural crest, and placode cells. These head structures are brought about initially by the neural induction between the organizer and the prospectiv...
Sakuma, Tetsushi; Yamamoto, Takashi
CANCER SCIENCE   109(12) 3679-3685   Dec 2018   [Refereed]
Genome editing includes various edits of the genome, such as short insertions and deletions, substitutions, and chromosomal rearrangements including inversions, duplications, and translocations. These variations are based on single or multiple DNA...
Nakagawa, Yoshiko; Sakuma, Tetsushi; Takeo, Toru; Nakagata, Naomi; Yamamoto, Takashi
EXPERIMENTAL ANIMALS   67(4) 535-543   Dec 2018   [Refereed]
Recently, genome editing in mouse zygotes has become convenient and scalable, in association with various technological developments and improvements such as novel nuclease tools, alternative delivery methods, and contemporary reproductive enginee...
Deguchi, Yutaka; Nishina, Takashi; Asano, Kenichi; Ohmuraya, Masaki; Nakagawa, Yoshiko; Nakagata, Naomi; Sakuma, Tetsushi; Yamamoto, Takashi; Araki, Kimi; Mikami, Tetuo; Tanaka, Masato; Nakano, Hiroyasu
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   505(2) 453-459   Oct 2018   [Refereed]
Interleukin (IL)-11 belongs to the members of the IL-6 family of cytokines and is involved in a variety of biological responses, including hematopoiesis, bone development, and carcinogenesis. However, the cellular sources of IL-11 and regulation o...
Miao, Wenyu; Sakai, Katsuya; Imamura, Ryu; Ito, Kenichiro; Suga, Hiroaki; Sakuma, Tetsushi; Yamamoto, Takashi; Matsumoto, Kunio
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES   19(10)    Oct 2018   [Refereed]
Non-native ligands for growth factor receptors with distinct chemical properties and different biological activities have the potential to become therapeutic applications. We previously generated MET/hepatocyte growth factor (HGF) receptor agonist...
Kunii, Atsushi; Hara, Yoshihiro; Takenaga, Mitsumasa; Hattori, Naoko; Fukazawa, Takuya; Ushijima, Toshikazu; Yamamoto, Takashi; Sakuma, Tetsushi
CRISPR JOURNAL   1(5) 337-347   Oct 2018   [Refereed]
In the past few years, several types of artificial transcriptional activator, based on CRISPR-Cas9, have been developed and refined. Of these, in synergistic activation mediator and SunTag systems, the effector proteins, expressed in trans, can be...
Tanigawa, Shunsuke; Islam, Mazharul; Sharmin, Sazia; Naganuma, Hidekazu; Yoshimura, Yasuhiro; Haque, Fahim; Era, Takumi; Nakazato, Hitoshi; Nakanishi, Koichi; Sakuma, Tetsushi; Yamamoto, Takashi; Kurihara, Hidetake; Taguchi, Atsuhiro; Nishinakamura, Ryuichi
STEM CELL REPORTS   11(3) 727-740   Sep 2018   [Refereed]
Mutations in the NPHS1 gene, which encodes NEPHRIN, cause congenital nephrotic syndrome, resulting from impaired slit diaphragm (SD) formation in glomerular podocytes. However, methods for SD reconstitution have been unavailable, thereby limiting ...
Nakade, Shota; Mochida, Keiji; Kunii, Atsushi; Nakamae, Kazuki; Aida, Tomomi; Tanaka, Kohichi; Sakamoto, Naoaki; Sakuma, Tetsushi; Yamamoto, Takashi
NATURE COMMUNICATIONS   9    Aug 2018   [Refereed]
Selective genome editing such as gene knock-in has recently been achieved by administration of chemical enhancer or inhibitor of particular DNA double-strand break (DSB) repair pathways, as well as overexpression of pathway-specific genes. In this...
Takashina, Tomoki; Koyama, Takayoshi; Nohara, Satoshi; Hasegawa, Masakatsu; Ishiguro, Akira; Iijima, Kenta; Lu, Jun; Shimura, Mari; Okamura, Tadashi; Sakuma, Tetsushi; Yamamoto, Takashi; Ishizaka, Yukihito
BIOMATERIALS   173 11-21   Aug 2018   [Refereed]
Cellular reprogramming is a promising technology in regenerative medicine, but most studies have been performed by using expression vectors. For future clinical applications, it is necessary to establish a system in which cell engineering can be m...
Saha, Liton Kumar; Kim, Sujin; Kang, Habyeong; Akter, Salma; Choi, Kyungho; Sakuma, Tetsushi; Yamamoto, Takashi; Sasanuma, Hiroyuki; Hirota, Kouji; Nakamura, Jun; Honma, Masamitsu; Takeda, Shunichi
ENVIRONMENTAL AND MOLECULAR MUTAGENESIS   59(6) 529-538   Jul 2018   [Refereed]
The micronucleus (MN) test has become an attractive tool both for evaluating the genotoxicity of test chemicals because of its ability to detect clastogenic and aneugenic events and for its convenience. As the MN assay has been mostly performed us...
Kawabe, Yoshinori; Komatsu, Shinya; Komatsu, Shodai; Murakami, Mai; Ito, Akira; Sakuma, Tetsushi; Nakamura, Takahiro; Yamamoto, Takashi; Kamihira, Masamichi
JOURNAL OF BIOSCIENCE AND BIOENGINEERING   125(5) 599-605   May 2018   [Refereed]
Chinese hamster ovary (CHO) cells have been used as host cells for the production of pharmaceutical proteins. For the high and stable production of target proteins, the transgene should be integrated into a suitable genomic locus of host cells. He...
Sakuma, Tetsushi; Mochida, Keiji; Nakade, Shota; Ezure, Toru; Minagawa, Sachi; Yamamoto, Takashi
GENES TO CELLS   23(4) 255-263   Apr 2018   [Refereed]
Single-cell cloning is an essential technique for establishing genome-edited cell clones mediated by programmable nucleases such as CRISPR-Cas9. However, residual genome-editing activity after single-cell cloning may cause heterogeneity in the clo...
Kim, Shin-Il; Matsumoto, Tomoko; Kagawa, Harunobu; Nakamura, Michiko; Hirohata, Ryoko; Ueno, Ayano; Ohishi, Maki; Sakuma, Tetsushi; Soga, Tomoyoshi; Yamamoto, Takashi; Woltjen, Knut
NATURE COMMUNICATIONS   9    Mar 2018   [Refereed]
Gene-edited induced pluripotent stem cells (iPSCs) provide relevant isogenic human disease models in patient-specific or healthy genetic backgrounds. Towards this end, gene targeting using antibiotic selection along with engineered point mutations...
Shukunami, Chisa; Takimoto, Aki; Nishizaki, Yuriko; Yoshimoto, Yuki; Tanaka, Seima; Miura, Shigenori; Watanabe, Hitomi; Sakuma, Tetsushi; Yamamoto, Takashi; Kondoh, Gen; Hiraki, Yuji
SCIENTIFIC REPORTS   8    Feb 2018   [Refereed]
Tenomodulin (Tnmd) is a type II transmembrane glycoprotein predominantly expressed in tendons and ligaments. We found that scleraxis (Scx), a member of the Twist-family of basic helix-loop-helix transcription factors, is a transcriptional activato...
Kuriyama, Sei; Tsuji, Tadahiro; Sakuma, Tetsushi; Yamamoto, Takashi; Tanaka, Masamistu
CELL DEATH DISCOVERY   4    Feb 2018   [Refereed]
The anti-apoptotic nature of cancer cells often impedes the effects of anti-cancer therapeutic agents. Multiple death signals influence mitochondria during apoptosis, and though many studies have attempted to elucidate these complicated pathways, ...
Nakade, Shota; Yamamoto, Takashi; Sakuma, Tetsushi
JOURNAL OF HUMAN GENETICS   63(2) 187-194   Feb 2018   [Refereed]
Cancer epigenetics is one of the most important research subjects in dissecting cancer mechanisms and therapeutic targets because the emergence and malignant transformation of various cancers are caused by unnatural expression of cancer-related ge...
Sato'o, Yusuke; Hisatsune, Junzo; Yu, Liansheng; Sakuma, Tetsushi; Yamamoto, Takashi; Sugai, Motoyuki
PLOS ONE   13(1)    Jan 2018   [Refereed]
Preparing the genetically modified organisms have required much time and labor, making it the rate-limiting step but CRISPR/Cas9 technology appearance has changed this difficulty. Although reports on CRISPR/Cas9 technology such as genome editing a...
Tsuda, Masataka; Cho, Kosai; Ooka, Masato; Shimizu, Naoto; Watanabe, Reiko; Yasui, Akira; Nakazawa, Yuka; Ogi, Tomoo; Harada, Hiroshi; Agama, Keli; Nakamura, Jun; Asada, Ryuta; Fujiike, Haruna; Sakuma, Tetsushi; Yamamoto, Takashi; Murai, Junko; Hiraoka, Masahiro; Koike, Kaoru; Pommier, Yves; Takeda, Shunichi; Hirota, Kouji
PLOS ONE   12(11)    Nov 2017   [Refereed]
ALC1/CHD1L is a member of the SNF2 superfamily of ATPases carrying a macrodomain that binds poly(ADP-ribose). Poly(ADP-ribose) polymerase (PARP) 1 and 2 synthesize poly(ADP-ribose) at DNA-strand cleavage sites, promoting base excision repair (BER)...
Kaku, Yusuke; Taguchi, Atsuhiro; Tanigawa, Shunsuke; Haque, Fahim; Sakuma, Tetsushi; Yamamoto, Takashi; Nishinakamura, Ryuichi
SCIENTIFIC REPORTS   7    Jul 2017   [Refereed]
The kidney is formed by reciprocal interactions between the nephron progenitor and the ureteric bud, the former of which gives rise to the epithelia of nephrons consisting of glomeruli and renal tubules. The transcription factor PAX2 is essential ...
Takayama, Kazuo; Igai, Keisuke; Hagihara, Yasuko; Hashimoto, Rina; Hanawa, Morifumi; Sakuma, Tetsushi; Tachibana, Masashi; Sakurai, Fuminori; Yamamoto, Takashi; Mizuguchi, Hiroyuki
NUCLEIC ACIDS RESEARCH   45(9) 5198-5207   May 2017   [Refereed]
Genome editing research of human ES/iPS cells has been accelerated by clustered regularly interspaced short palindromic repeats/CRISPR-associated 9 (CRISPR/Cas9) and transcription activator-like effector nucleases (TALEN) technologies. However, th...
Nakagawa, Yoshiko; Sakuma, Tetsushi; Nishimichi, Norihisa; Yokosaki, Yasuyuki; Takeo, Toru; Nakagata, Naomi; Yamamoto, Takashi
BIOLOGY OPEN   6(5) 706-713   May 2017   [Refereed]
Robust reproductive engineering techniques are required for the efficient and rapid production of genetically modified mice. We have reported the efficient production of genome-edited mice using reproductive engineering techniques, such as ultra-s...
Yoshida, Keita; Nakahata, Azusa; Treen, Nicholas; Sakuma, Tetsushi; Yamamoto, Takashi; Sasakura, Yasunori
DEVELOPMENT   144(9) 1629-1634   May 2017   [Refereed]
The chordate pharynx, possessing gill slits and the endostyle, is a complex of multiple tissues that are highly organized along the anterior-posterior (AP) axis. Although Hox genes show AP coordinated expression in the pharyngeal endoderm, tissue-...
Yoshida, Keita; Hozumi, Akiko; Treen, Nicholas; Sakuma, Tetsushi; Yamamoto, Takashi; Shirae-Kurabayashi, Maki; Sasakura, Yasunori
DEVELOPMENTAL BIOLOGY   423(2) 111-125   Mar 2017   [Refereed]
The ascidian Ciona intestinalis has a high regeneration capacity that enables the regeneration of artificially removed primordial germ cells (PGCs) from somatic cells. We utilized PGC regeneration to establish efficient methods of germ line mutage...
Mizutani, Osamu; Arazoe, Takayuki; Toshida, Kenji; Hayashi, Risa; Ohsato, Shuichi; Sakuma, Tetsushi; Yamamoto, Takashi; Kuwata, Shigeru; Yamada, Osamu
JOURNAL OF BIOSCIENCE AND BIOENGINEERING   123(3) 287-293   Mar 2017   [Refereed]
Transcription activator-like effector nucleases (TALENs), which can generate DNA double-strand breaks at specific sites in the desired genome locus, have been used in many organisms as a tool for genome editing. In Aspergilli, including Aspergillu...
Nakade, Shota; Yamamoto, Takashi; Sakuma, Tetsushi
BIOENGINEERED   8(3) 265-273   2017   [Refereed]
Since the rapid emergence of clustered regulatory interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein 9 (Cas9) system, developed as a genome engineering tool in 2012-2013, most researchers in the life science field have had a ...
Nakamae, Kazuki; Nishimura, Yuki; Takenaga, Mitsumasa; Nakade, Shota; Sakamoto, Naoaki; Ide, Hiroshi; Sakuma, Tetsushi; Yamamoto, Takashi
BIOENGINEERED   8(3) 302-308   2017   [Refereed]
The emerging genome editing technology has enabled the creation of gene knock-in cells easily, efficiently, and rapidly, which has dramatically accelerated research in the field of mammalian functional genomics, including in humans. We recently de...
Nakamura, Minako; Kumrungsee, Thanutchaporn; Sakuma, Tetsushi; Yamamoto, Takashi; Yanaka, Noriyuki
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   81(11) 2164-2167   2017   [Refereed]
In this study, we investigated the physiological function of glycerophosphodiesterase 5 (GDE5) in the proliferation of NIH3T3 fibroblasts. We used transcription activator-like effector nuclease (TALEN) in NIH3T3 cells with an intron targeting-medi...
Sasakura, Yasunori; Ogura, Yosuke; Treen, Nicholas; Yokomori, Rui; Park, Sung-Joon; Nakai, Kenta; Saiga, Hidetoshi; Sakuma, Tetsushi; Yamamoto, Takashi; Fujiwara, Shigeki; Yoshida, Keita
PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES   283(1845)    Dec 2016   [Refereed]
The horizontal transfer of genes between distantly related organisms is undoubtedly a major factor in the evolution of novel traits. Because genes are functionless without expression, horizontally transferred genes must acquire appropriate transcr...
Toyonaga, Kenji; Torigoe, Shota; Motomura, Yoshitomo; Kamichi, Takane; Hayashi, Jennifer M.; Morita, Yasu S.; Noguchi, Naoto; Chuma, Yasushi; Kiyohara, Hideyasu; Matsuo, Kazuhiro; Tanaka, Hiroshi; Nakagawa, Yoshiko; Sakuma, Tetsushi; Ohmuraya, Masaki; Yamamoto, Takashi; Umemura, Masayuki; Matsuzaki, Goro; Yoshikai, Yasunobu; Yano, Ikuya; Miyamoto, Tomofumi; Yamasaki, Sho
IMMUNITY   45(6) 1245-1257   Dec 2016   [Refereed]
Phosphatidyl-inositol mannosides (PIM) are glycolipids unique to mycobacteria and other related bacteria that stimulate host immune responses and are implicated in mycobacteria pathogenicity. Here, we found that the FcRg-coupled C-type lectin rece...
Sasaki, Takako; Hanisch, Franz-Georg; Deutzmann, Rainer; Sakai, Lynn Y.; Sakuma, Tetsushi; Miyamoto, Tatsuo; Yamamoto, Takashi; Hannappel, Ewald; Chu, Mon-Li; Lanig, Harald; von der Mark, Klaus
MATRIX BIOLOGY   56 132-149   Dec 2016   [Refereed]
Fibulin-4 is a 60 kDa calcium binding glycoprotein that has an important role in development and integrity of extracellular matrices. It interacts with elastin, fibrillin-1 and collagen IV as well as with lysyl oxidases and is involved in elastoge...
Aida, Tomomi; Nakade, Shota; Sakuma, Tetsushi; Izu, Yayoi; Oishi, Ayu; Mochida, Keiji; Ishikubo, Harumi; Usami, Takako; Aizawa, Hidenori; Yamamoto, Takashi; Tanaka, Kohichi
BMC GENOMICS   17    Nov 2016   [Refereed]
Background: Although CRISPR/Cas enables one-step gene cassette knock-in, assembling targeting vectors containing long homology arms is a laborious process for high-throughput knock-in. We recently developed the CRISPR/Cas-based precise integration...
Tochio, Naoya; Umehara, Kohei; Uewaki, Jun-ichi; Flechsig, Holger; Kondo, Masaharu; Dewa, Takehisa; Sakuma, Tetsushi; Yamamoto, Takashi; Saitoh, Takashi; Togashi, Yuichi; Tate, Shin-ichi
SCIENTIFIC REPORTS   6    Nov 2016   [Refereed]
Transcription activator-like effector (TALE) nuclease (TALEN) is widely used as a tool in genome editing. The DNA binding part of TALEN consists of a tandem array of TAL-repeats that form a right-handed superhelix. Each TAL-repeat recognises a spe...
Sakuma, Tetsushi; Masaki, Keiichi; Abe-Chayama, Hiromi; Mochida, Keiji; Yamamoto, Takashi; Chayama, Kazuaki
GENES TO CELLS   21(11) 1253-1262   Nov 2016   [Refereed]
CRISPR-Cas9-mediated genome-editing technology contributes not only to basic genomic studies but also to clinical studies such as genetic correction and virus inactivation. Hepatitis B virus (HBV) is a major target for potential application of CRI...
Nakagawa, Yoshiko; Sakuma, Tetsushi; Nishimichi, Norihisa; Yokosaki, Yasuyuki; Yanaka, Noriyuki; Takeo, Toru; Nakagata, Naomi; Yamamoto, Takashi
BIOLOGY OPEN   5(8) 1142-1148   Aug 2016   [Refereed]
Current advances in producing genetically modified mice using genome-editing technologies have indicated the need for improvement of limiting factors including zygote collection for microinjection and their cryopreservation. Recently, we developed...
Generation of a Nonhuman Primate Model of Severe Combined Immunodeficiency Using Highly Efficient Genome Editing
Sato, Kenya; Oiwa, Ryo; Kumita, Wakako; Henry, Rachel; Sakuma, Tetsushi; Ito, Ryoji; Nozu, Ryoko; Inoue, Takashi; Katano, Ikumi; Sato, Kengo; Okahara, Norio; Okahara, Junko; Shimizu, Yoshihisa; Yamamoto, Masafumi; Hanazawa, Kisaburo; Kawakami, Takao; Kametani, Yoshie; Suzuki, Ryuji; Takahashi, Takeshi; Weinstein, Edward J.; Yamamoto, Takashi; Sakakibara, Yasubumi; Habu, Sonoko; Hata, Jun-ichi; Okano, Hideyuki; Sasaki, Erika
CELL STEM CELL   19(1) 127-138   Jul 2016   [Refereed]
Recent advances in genome editing have facilitated the generation of nonhuman primate (NHP) models, with potential to unmask the complex biology of human disease not revealed by rodent models. However, their broader use is hindered by the challeng...
Involvement of aspartoacylase in tremor expression in rats
Nishitani, Ai; Tanaka, Miyuu; Shimizu, Saki; Kunisawa, Naofumi; Yokoe, Mayuko; Yoshida, Yusaku; Suzuki, Toshiro; Sakuma, Tetsushi; Yamamoto, Takashi; Kuwamura, Mitsuru; Takenaka, Shigeo; Ohno, Yukihiro; Kuramoto, Takashi
EXPERIMENTAL ANIMALS   65(3) 293-301   Jul 2016   [Refereed]
Essential tremor (ET) is a common movement disorder with a poorly understood etiology. The TRM/Kyo mutant rat, showing spontaneous tremor, is an animal model of ET. Recently, we demonstrated that tremors in these rats emerge when two mutant loci, ...
Sharmin, Sazia; Taguchi, Atsuhiro; Kaku, Yusuke; Yoshimura, Yasuhiro; Ohmori, Tomoko; Sakuma, Tetsushi; Mukoyama, Masashi; Yamamoto, Takashi; Kurihara, Hidetake; Nishinakamura, Ryuichi
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY   27(6) 1778-1791   Jun 2016   [Refereed]
Glomerular podocytes express proteins, such as nephrin, that constitute the slit diaphragm, thereby contributing to the filtration process in the kidney. Glomerular development has been analyzed mainly in mice, whereas analysis of human kidney dev...
Takata, Nozomu; Sakakura, Eriko; Kasukawa, Takeya; Sakuma, Tetsushi; Yamamoto, Takashi; Sasai, Yoshiki
HUMAN GENE THERAPY   27(6) 436-450   Jun 2016   [Refereed]
The epiblast (foremost embryonic ectoderm) generates all three germ layers and therefore has crucial roles in the formation of all mammalian body cells. However, regulation of epiblast gene expression is poorly understood because of the difficulty...
Banno, Kimihiko; Omori, Sayaka; Hirata, Katsuya; Nawa, Nobutoshi; Nakagawa, Natsuki; Nishimura, Ken; Ohtaka, Manami; Nakanishi, Mahito; Sakuma, Tetsushi; Yamamoto, Takashi; Toki, Tsutomu; Ito, Etsuro; Yamamoto, Toshiyuki; Kokubu, Chikara; Takeda, Junji; Taniguchi, Hidetoshi; Arahori, Hitomi; Wada, Kazuko; Kitabatake, Yasuji; Ozono, Keiichi
CELL REPORTS   15(6) 1228-1241   May 2016   [Refereed]
Chromosomal aneuploidy and specific gene mutations are recognized early hallmarks of many oncogenic processes. However, the net effect of these abnormalities has generally not been explored. We focused on transient myeloproliferative disorder (TMD...
Marsan, Elise; Ishida, Saeko; Schramm, Adrien; Weckhuysen, Sarah; Muraca, Giuseppe; Lecas, Sarah; Liang, Ning; Treins, Caroline; Pende, Mario; Roussel, Delphine; Le Van Quyen, Michel; Mashimo, Tomoji; Kaneko, Takehito; Yamamoto, Takashi; Sakuma, Tetsushi; Mahon, Severine; Miles, Richard; Leguern, Eric; Charpier, Stephane; Baulac, Stephanie
NEUROBIOLOGY OF DISEASE   89 180-189   May 2016   [Refereed]
DEP-domain containing 5 (DEPDC5), encoding a repressor of the mechanistic target of rapamycin complex 1 (mTORC1) signaling pathway, has recently emerged as a major gene mutated in familial focal epilepsies and focal cortical dysplasia. Here we est...
Nakahara, Yoshinari; Muto, Akihiko; Hirabayashi, Ryo; Sakuma, Tetsushi; Yamamoto, Takashi; Kume, Shoen; Kikuchi, Yutaka
GENES TO CELLS   21(5) 492-504   May 2016   [Refereed]
The adenohypophysis (AH) consists of six distinct types of hormone-secreting cells. In zebrafish, although proper differentiation of all AH cell types has been shown to require Notch signaling within a period of 14-16h postfertilization (hpf), the...
Nii, Takenobu; Kohara, Hiroshi; Marumoto, Tomotoshi; Sakuma, Tetsushi; Yamamoto, Takashi; Tani, Kenzaburo
BIORESEARCH OPEN ACCESS   5(1) 127-136   May 2016   [Refereed]
Efficient gene transfer into human pluripotent stem cells (hPSCs) holds great promise for regenerative medicine and pharmaceutical development. In the past decade, various methods were developed for gene transfer into hPSCs; however, hPSCs form ti...
Ichiyanagi, Naoki; Fujimori, Koki; Yano, Masato; Ishihara-Fujisaki, Chikako; Sone, Takefumi; Akiyama, Tetsuya; Okada, Yohei; Akamatsu, Wado; Matsumoto, Takuya; Ishikawa, Mitsuru; Nishimoto, Yoshinori; Ishihara, Yasuharu; Sakuma, Tetsushi; Yamamoto, Takashi; Tsuiji, Hitomi; Suzuki, Naoki; Warita, Hitoshi; Aoki, Masashi; Okano, Hideyuki
STEM CELL REPORTS   6(4) 496-510   Apr 2016   [Refereed]
Amyotrophic lateral sclerosis (ALS) is a late-onset motor neuron disorder. Although its neuropathology is well understood, the cellular and molecular mechanisms are yet to be elucidated due to limitations in the currently available human genetic d...
Suzuki, Miyuki; Takagi, Chiyo; Miura, Shinichirou; Sakane, Yuto; Suzuki, Makoto; Sakuma, Tetsushi; Sakamoto, Naoaki; Endo, Tetsuya; Kamei, Yasuhiro; Sato, Yuko; Kimura, Hiroshi; Yamamoto, Takashi; Ueno, Naoto; Suzuki, Ken-ichi T.
GENES TO CELLS   21(4) 358-369   Apr 2016   [Refereed]
Xenopus laevis tadpoles can completely regenerate their appendages, such as tail and limbs, and therefore provide a unique model to decipher the molecular mechanisms of organ regeneration in vertebrates. Epigenetic modifications are likely to be i...
Guo, Long; Yamashita, Hiroshi; Kou, Ikuyo; Takimoto, Aki; Meguro-Horike, Makiko; Horike, Shin-ichi; Sakuma, Tetsushi; Miura, Shigenori; Adachi, Taiji; Yamamoto, Takashi; Ikegawa, Shiro; Hiraki, Yuji; Shukunami, Chisa
PLOS GENETICS   12(1)    Jan 2016   [Refereed]
Previously, we identified an adolescent idiopathic scoliosis susceptibility locus near human ladybird homeobox 1 (LBX1) and FLJ41350 by a genome-wide association study. Here, we characterized the associated non-coding variant and investigated the ...
Rao, Shengbin; Fujimura, Tatsuya; Matsunari, Hitomi; Sakuma, Tetsushi; Nakano, Kazuaki; Watanabe, Masahito; Asano, Yoshinori; Kitagawa, Eri; Yamamoto, Takashi; Nagashima, Hiroshi
MOLECULAR REPRODUCTION AND DEVELOPMENT   83(1) 61-70   Jan 2016   [Refereed]
Myostatin (MSTN) is a negative regulator of myogenesis, and disruption of its function causes increased muscle mass in various species. Here, we report the generation of MSTN-knockout (KO) pigs using genome editing technology combined with somatic...
Sakuma, Tetsushi; Nakade, Shota; Sakane, Yuto; Suzuki, Ken-Ichi T; Yamamoto, Takashi
NATURE PROTOCOLS   11(1) 118-133   Jan 2016   [Refereed]
Programmable nucleases enable engineering of the genome by utilizing endogenous DNA double-strand break (DSB) repair pathways. Although homologous recombination (HR)-mediated gene knock-in is well established, it cannot necessarily be applied in e...
Nguyen Ngoc Hoa; Akagawa, Remi; Yamasaki, Tomomi; Hirota, Kouji; Sasa, Kentaro; Natsume, Toyoaki; Kobayashi, Junya; Sakuma, Tetsushi; Yamamoto, Takashi; Komatsu, Kenshi; Kanemaki, Masato T.; Pommier, Yves; Takeda, Shunichi; Sasanuma, Hiroyuki
GENES TO CELLS   20(12) 1059-1076   Dec 2015   [Refereed]
Homologous recombination (HR) is initiated by double-strand break (DSB) resection, during which DSBs are processed by nucleases to generate 30 single-strand DNA. DSB resection is initiated by CtIP and Mre11 followed by long-range resection by Dna2...
Miyamoto, Kei; Suzuki, Ken-ichi T.; Suzuki, Miyuki; Sakane, Yuto; Sakuma, Tetsushi; Herberg, Sarah; Simeone, Angela; Simpson, David; Jullien, Jerome; Yamamoto, Takashi; Gurdon, J. B.
PLOS ONE   10(11)    Nov 2015   [Refereed]
Recent advances in genome editing using programmable nucleases have revolutionized gene targeting in various organisms. Successful gene knock-out has been shown in Xenopus, a widely used model organism, although a system enabling less mosaic knock...
Sakuma, Tetsushi; Takenaga, Mitsumasa; Kawabe, Yoshinori; Nakamura, Takahiro; Kamihira, Masamichi; Yamamoto, Takashi
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES   16(10) 23849-23866   Oct 2015   [Refereed]
Gene knock-in techniques have rapidly evolved in recent years, along with the development and maturation of genome editing technology using programmable nucleases. We recently reported a novel strategy for microhomology-mediated end-joining-depend...
Nakade, Shota; Sakuma, Tetsushi; Sakane, Yuto; Hara, Yoshihiro; Kurabayashi, Atsushi; Kashiwagi, Keiko; Kashiwagi, Akihiko; Yamamoto, Takashi; Obara, Masanobu
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL   51(9) 879-884   Oct 2015   [Refereed]
Transcription activator-like effector nucleases (TALENs) have previously been used for targeted genome editing in various organisms including Xenopus laevis. However, because of genomic polyploidization, X. laevis usually possess homeologous genes...
Fujiwara, Miwako; Nagatomo, Azusa; Tsuda, Megumi; Obata, Shuichi; Sakuma, Tetsushi; Yamamoto, Takashi; Suzuki, Shintaro T.
JOURNAL OF BIOCHEMISTRY   158(4) 339-353   Oct 2015   [Refereed]
The role of the juxtamembrane region of the desmocollin-2 cytoplasmic domain in desmosome formation was investigated by using gene knockout and reconstitution experiments. When a deletion construct of the desmocollin-2 juxtamembrane region was exp...
Sasaki, Kotaro; Yokobayashi, Shihori; Nakamura, Tomonori; Okamoto, Ikuhiro; Yabuta, Yukihiro; Kurimoto, Kazuki; Ohta, Hiroshi; Moritoki, Yoshinobu; Iwatani, Chizuru; Tsuchiya, Hideaki; Nakamura, Shinichiro; Sekiguchi, Kiyotoshi; Sakuma, Tetsushi; Yamamoto&
CELL STEM CELL   17(2) 178-194   Aug 2015   [Refereed]
Mechanisms underlying human germ cell development are unclear, partly due to difficulties in studying human embryos and lack of suitable experimental systems. Here, we show that human induced pluripotent stem cells (hiPSCs) differentiate into inci...
Keka, Islam Shamima; Mohiuddin; Maede, Yuko; Rahman, Md Maminur; Sakuma, Tetsushi; Honma, Masamitsu; Yamamoto, Takashi; Takeda, Shunichi; Sasanuma, Hiroyuki
NUCLEIC ACIDS RESEARCH   43(13) 6359-6372   Jul 2015   [Refereed]
Smarcal1 is a SWI/SNF-family protein with an ATPase domain involved in DNA-annealing activities and a binding site for the RPA single-strand-DNA-binding protein. Although the role played by Smarcal1 in the maintenance of replication forks has been...
Sato, Takuya; Sakuma, Tetsushi; Yokonishi, Tetsuhiro; Katagiri, Kumiko; Kamimura, Satoshi; Ogonuki, Narumi; Ogura, Atsuo; Yamamoto, Takashi; Ogawa, Takehiko
STEM CELL REPORTS   5(1) 75-82   Jul 2015   [Refereed]
Mouse spermatogonial stem cells (SSCs) can be cultured for multiplication and maintained for long periods while preserving their spermatogenic ability. Although the cultured SSCs, named germline stem (GS) cells, are targets of genome modification,...
Kawai, Narudo; Ogura, Yosuke; Ikuta, Tetsuro; Saiga, Hidetoshi; Hamada, Mayuko; Sakuma, Tetsushi; Yamamoto, Takashi; Satoh, Nori; Sasakura, Yasunori
DEVELOPMENTAL BIOLOGY   403(1) 43-56   Jul 2015   [Refereed]
Hox cluster genes play crucial roles in development of the metazoan antero-posterior axis. Functions of Hox genes in patterning the central nervous system and limb buds are well known. They are also expressed in chordate endodermal tissues, where ...
Arazoe, Takayuki; Ogawa, Tetsuo; Miyoshi, Kennosuke; Yamato, Tohru; Ohsato, Shuichi; Sakuma, Tetsushi; Yamamoto, Takashi; Arie, Tsutomu; Kuwata, Shigeru
BIOTECHNOLOGY AND BIOENGINEERING   112(7) 1335-1342   Jul 2015   [Refereed]
Genetic manipulation is key to unraveling gene functions and creating genetically modified strains of microbial organisms. Recently, engineered nucleases that can generate DNA double-strand breaks (DSBs) at a specific site in the desired locus wit...
Hara, Satoshi; Tamano, Moe; Yamashita, Satoshi; Kato, Tomoko; Saito, Takeshi; Sakuma, Tetsushi; Yamamoto, Takashi; Inui, Masafumi; Takada, Shuji
SCIENTIFIC REPORTS   5    Jun 2015   [Refereed]
Genome editing, which introduces mutations in genes of interest using artificial DNA nucleases such as the ZFN, TALEN, and CRISPR/Cas9 systems in living cells, is a useful tool for generating mutant animals. Although CRISPR/Cas9 provides advantage...
Nakagawa, Yoshiko; Sakuma, Tetsushi; Sakamoto, Takuya; Ohmuraya, Masaki; Nakagata, Naomi; Yamamoto, Takashi
BMC BIOTECHNOLOGY   15    May 2015   [Refereed]
Background: Clustered regulatory interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9)-mediated genome editing permits the rapid production of genetically engineered mice. To make the most of this innovative technology,...
Aida, Tomomi; Chiyo, Keiho; Usami, Takako; Ishikubo, Harumi; Imahashi, Risa; Wada, Yusaku; Tanaka, Kenji F.; Sakuma, Tetsushi; Yamamoto, Takashi; Tanaka, Kohichi
GENOME BIOLOGY   16    Apr 2015   [Refereed]
Although the CRISPR/Cas system has enabled one-step generation of knockout mice, low success rates of cassette knock-in limit its application range. Here we show that cloning-free, direct nuclear delivery of Cas9 protein complex with chemically sy...
Ebina, Hirotaka; Kanemura, Yuka; Misawa, Naoko; Sakuma, Tetsushi; Kobayashi, Tomoko; Yamamoto, Takashi; Koyanagi, Yoshio
PLOS ONE   10(3)    Mar 2015   [Refereed]
DNA-editing technology has made it possible to rewrite genetic information in living cells. Human immunodeficiency virus (HIV) provirus, an integrated form of viral complementary DNA in host chromosomes, could be a potential target for this techno...
Hisano, Yu; Sakuma, Tetsushi; Nakade, Shota; Ohga, Rie; Ota, Satoshi; Okamoto, Hitoshi; Yamamoto, Takashi; Kawahara, Atsuo
SCIENTIFIC REPORTS   5    Mar 2015   [Refereed]
The CRISPR/Cas9 system provides a powerful tool for genome editing in various model organisms, including zebrafish. The establishment of targeted gene-disrupted zebrafish (knockouts) is readily achieved by CRISPR/Cas9-mediated genome modification....
Miyamoto, Tatsuo; Hosoba, Kosuke; Ochiai, Hiroshi; Royba, Ekaterina; Izumi, Hideki; Sakuma, Tetsushi; Yamamoto, Takashi; Dynlacht, Brian David; Matsuura, Shinya
CELL REPORTS   10(5) 664-673   Feb 2015   [Refereed]
The primary cilium is an antenna-like, microtubule-based organelle on the surface of most vertebrate cells for receiving extracellular information. Although primary cilia form in the quiescent phase, ciliary disassembly occurs when quiescent cells...
Choi, Jinyoung; Suzuki, Ken-ichi T.; Sakuma, Tetsushi; Shewade, Leena; Yamamoto, Takashi; Buchholz, Daniel R.
ENDOCRINOLOGY   156(2) 735-744   Feb 2015   [Refereed]
Thyroid hormone (TH) receptor (TR) expression begins early in development in all vertebrates when circulating TH levels are absent or minimal, yet few developmental roles for unliganded TRs have been established. Unliganded TRs are expected to rep...
Li, Hongmei Lisa; Fujimoto, Naoko; Sasakawa, Noriko; Shirai, Saya; Ohkame, Tokiko; Sakuma, Tetsushi; Tanaka, Michihiro; Amano, Naoki; Watanabe, Akira; Sakurai, Hidetoshi et al.
STEM CELL REPORTS   4(1) 143-154   Jan 2015   [Refereed]
Duchenne muscular dystrophy (DMD) is a severe muscle-degenerative disease caused by a mutation in the dystrophin gene. Genetic correction of patient-derived induced pluripotent stem cells (iPSCs) by TALENs or CRISPR-Cas9 holds promise for DMD gene...
Epithelial DLD-1 Cells with Disrupted E-cadherin Gene Retain the Ability to Form Cell Junctions and Apico-basal Polarity
Fujiwara, Miwako; Fujimura, Kihito; Obata, Shuichi; Yanagibashi, Ryo; Sakuma, Tetsushi; Yamamoto, Takashi; Suzuki, Shintaro T.
CELL STRUCTURE AND FUNCTION   40(2) 79-94   2015   [Refereed]
Gene editing methods were applied to the study of E-cadherin function in epithelial cells. The Ecadherin gene in epithelial DLD-1 cells was ablated using TALEN. The resultant cells showed round fibroblastlike morphology and had almost no Ca2+-depe...
Ochiai, Hiroshi; Sugawara, Takeshi; Sakuma, Tetsushi; Yamamoto, Takashi
SCIENTIFIC REPORTS   4    Nov 2014   [Refereed]
Mouse embryonic stem cells (mESCs) are self-renewing and capable of differentiating into any of the three germ layers. An interesting feature of mESCs is the presence of cell-to-cell heterogeneity in gene expression that may be responsible for cel...
Hiruta, Chizue; Ogino, Yukiko; Sakuma, Tetsushi; Toyota, Kenji; Miyagawa, Shinichi; Yamamoto, Takashi; Iguchi, Taisen
BMC BIOTECHNOLOGY   14    Nov 2014   [Refereed]
Background: The cosmopolitan microcrustacean Daphnia pulex provides a model system for both human health research and monitoring ecosystem integrity. It is the first crustacean to have its complete genome sequenced, an unprecedented ca. 36% of whi...
Nakade, Shota; Tsubota, Takuya; Sakane, Yuto; Kume, Satoshi; Sakamoto, Naoaki; Obara, Masanobu; Daimon, Takaaki; Sezutsu, Hideki; Yamamoto, Takashi; Sakuma, Tetsushi et al.
NATURE COMMUNICATIONS   5    Nov 2014   [Refereed]
Genome engineering using programmable nucleases enables homologous recombination (HR)-mediated gene knock-in. However, the labour used to construct targeting vectors containing homology arms and difficulties in inducing HR in some cell type and or...
Kaneko, Takehito; Sakuma, Tetsushi; Yamamoto, Takashi; Mashimo, Tomoji
SCIENTIFIC REPORTS   4    Oct 2014   [Refereed]
Engineered endonucleases, such as zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated (Cas) system, provide a powerful ...
Sawai, Satoru; Ohyama, Kiyoshi; Yasumoto, Shuhei; Seki, Hikaru; Sakuma, Tetsushi; Yamamoto, Takashi; Takebayashi, Yumiko; Kojima, Mikiko; Sakakibara, Hitoshi; Aoki, Toshio et al.
PLANT CELL   26(9) 3763-3774   Sep 2014   [Refereed]
Potatoes (Solanum tuberosum) contain alpha-solanine and alpha-chaconine, two well-known toxic steroidal glycoalkaloids (SGAs). Sprouts and green tubers accumulate especially high levels of SGAs. Although SGAs were proposed to be biosynthesized fro...
Kazuki, Yasuhiro; Yakura, Yuwna; Abe, Satoshi; Osaki, Mitsuhiko; Kajitani, Naoyo; Kazuki, Kanako; Takehara, Shoko; Honma, Kazuhisa; Suemori, Hirofumi; Yamazaki, Satoshi et al.
SCIENTIFIC REPORTS   4    Aug 2014   [Refereed]
Infants with Down syndrome (DS) are at a high risk of developing transient abnormal myelopoiesis (TAM). A GATA1 mutation leading to the production of N-terminally truncated GATA1 (GATA1s) in early megakaryocyte/erythroid progenitors is linked to t...
Ninagawa, Satoshi; Okada, Tetsuya; Sumitomo, Yoshiki; Kamiya, Yukiko; Kato, Koichi; Horimoto, Satoshi; Ishikawa, Tokiro; Takeda, Shunichi; Sakuma, Tetsushi; Yamamoto, Takashi et al.
JOURNAL OF CELL BIOLOGY   206(3) 347-356   Aug 2014   [Refereed]
Glycoproteins misfolded in the endoplasmic reticulum (ER) are subjected to ER-associated glycoprotein degradation (gpERAD) in which Htm1 -mediated mannose trimming from the oligosaccharide Man(8)GIcNAc(2) to Man(7)GIcNAc(2) is the rate-limiting st...
Yasue, Akihiro; Mitsui, Silvia Naomi; Watanabe, Takahito; Sakuma, Tetsushi; Oyadomari, Seiichi; Yamamoto, Takashi; Noji, Sumihare; Mito, Taro; Tanaka, Eiji
SCIENTIFIC REPORTS   4    Jul 2014   [Refereed]
Since the establishment of embryonic stem (ES) cell lines, the combined use of gene targeting with homologous recombination has aided in elucidating the functions of various genes. However, the ES cell technique is inefficient and time-consuming. ...
Application of Oocyte Cryopreservation Technology in TALEN-Mediated Mouse Genome Editing
Nakagawa, Yoshiko; Sakuma, Tetsushi; Nakagata, Naomi; Yamasaki, Sho; Takeda, Naoki; Ohmuraya, Masaki; Yamamoto, Takashi
EXPERIMENTAL ANIMALS   63(3) 349-355   Jul 2014   [Refereed]
Reproductive engineering techniques, such as in vitro fertilization (IVF) and cryopreservation of embryos or spermatozoa, are essential for preservation, reproduction, and transportation of genetically engineered mice. However, it has not yet been...
Sakuma, Tetsushi; Nishikawa, Ayami; Kume, Satoshi; Chayama, Kazuaki; Yamamoto, Takashi
SCIENTIFIC REPORTS   4    Jun 2014   [Refereed]
CRISPR/Cas9-mediated genome editing is a next-generation strategy for genetic modifications, not only for single gene targeting, but also for multiple targeted mutagenesis. To make the most of the multiplexity of CRISPR/Cas9, we established a syst...
Yoshida, Keita; Treen, Nicholas; Hozumi, Akiko; Sakuma, Tetsushi; Yamamoto, Takashi; Sasakura, Yasunori
GENESIS   52(5) 431-439   May 2014   [Refereed]
Targeted mutagenesis of genes-of-interest, or gene-knockout, is a powerful method to address the functions of genes. Engineered nucleases have enabled this approach in various organisms because of their ease of use. The ascidian Ciona intestinalis...
Tokumasu, Daisuke; Sakuma, Tetsushi; Hayashi, Yoko; Hosoi, Sayaka; Hiyama, Eiso; Yamamoto, Takashi
GENES TO CELLS   19(5) 419-431   May 2014   [Refereed]
Transcription activator-like effector nuclease (TALEN)-mediated genome editing is a powerful technique for analyzing gene functions in various cells and organisms. At target loci, TALENs can not only introduce short insertions and deletions, but a...
Ochiai, Hiroshi; Miyamoto, Tatsuo; Kanai, Akinori; Hosoba, Kosuke; Sakuma, Tetsushi; Kudo, Yoshiki; Asami, Keiko; Ogawa, Atsushi; Watanabe, Akihiro; Kajii, Tadashi et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   111(4) 1461-1466   Jan 2014   [Refereed]
Cancer-prone syndrome of premature chromatid separation with mosaic variegated aneuploidy [PCS (MVA) syndrome] is a rare autosomal recessive disorder characterized by constitutional aneuploidy and a high risk of childhood cancer. We previously rep...
Treen, Nicholas; Yoshida, Keita; Sakuma, Tetsushi; Sasaki, Haruka; Kawai, Narudo; Yamamoto, Takashi; Sasakura, Yasunori
DEVELOPMENT   141(2) 481-487   Jan 2014   [Refereed]
Custom designed nucleases can simplify gene targeting experiments and have the potential to allow these techniques to be performed in a wide range of organisms. Transcriptional activator-like effector nucleases (TALENs) are starting to fulfill thi...
Sugi, Takuma; Sakuma, Tetsushi; Ohtani, Yasuko; Yamamoto, Takashi
DEVELOPMENT GROWTH & DIFFERENTIATION   56(1) 78-85   Jan 2014   [Refereed]
Targeted genome editing using transcription activator-like effector nuclease (TALEN) and clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 systems has recently emerged as a potentially powerful method for creating locus-speci...
Sakane, Yuto; Sakuma, Tetsushi; Kashiwagi, Keiko; Kashiwagi, Akihiko; Yamamoto, Takashi; Suzuki, Ken-ichi T.
DEVELOPMENT GROWTH & DIFFERENTIATION   56(1) 108-114   Jan 2014   [Refereed]
Transcription activator-like effector nucleases (TALENs) have been extensively used in genome editing in various organisms. In some cases, however, it is difficult to efficiently disrupt both paralogous genes using a single pair of TALENs in Xenop...
Hosoi, Sayaka; Sakuma, Tetsushi; Sakamoto, Naoaki; Yamamoto, Takashi
DEVELOPMENT GROWTH & DIFFERENTIATION   56(1) 92-97   Jan 2014   [Refereed]
Genome editing with engineered nucleases such as zinc-finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs) has been reported in various animals. We previously described ZFN-mediated targeted mutagenesis and insertio...
Hayashi, Toshinori; Sakamoto, Kousuke; Sakuma, Tetsushi; Yokotani, Naoki; Inoue, Takeshi; Kawaguchi, Eri; Agata, Kiyokazu; Yamamoto, Takashi; Takeuchi, Takashi
DEVELOPMENT GROWTH & DIFFERENTIATION   56(1) 115-121   Jan 2014   [Refereed]
Regeneration of a lost tissue in an animal is an important issue. Although regenerative studies have a history of research spanning more than a century, the gene functions underlying regulation of the regeneration are mostly unclear. Analysis of k...
Kondo, Takefumi; Sakuma, Tetsushi; Wada, Housei; Akimoto-Kato, Ai; Yamamoto, Takashi; Hayashi, Shigeo
DEVELOPMENT GROWTH & DIFFERENTIATION   56(1) 86-91   Jan 2014   [Refereed]
We report here a case study of TALEN-induced gene knock out of the trachealess gene of Drosophila. Two pairs of TALEN constructs caused targeted mutation in the germ line of 39% and 17% of injected animals, respectively. In the extreme case 100% o...
Sakuma, Tetsushi; Woltjen, Knut
DEVELOPMENT GROWTH & DIFFERENTIATION   56(1) 2-13   Jan 2014   [Refereed]
Genome editing with engineered endonucleases is rapidly becoming a staple method in developmental biology studies. Engineered nucleases permit random or designed genomic modification at precise loci through the stimulation of endogenous double-str...
Screening Methods to Identify TALEN-Mediated Knockout Mice
Nakagawa, Yoshiko; Yamamoto, Takashi; Suzuki, Ken-Ichi; Araki, Kimi; Takeda, Naoki; Ohmuraya, Masaki; Sakuma, Tetsushi
EXPERIMENTAL ANIMALS   63(1) 79-84   Jan 2014   [Refereed]
Genome editing with site-specific nucleases, such as zinc-finger nucleases or transcription activator-like effector nucleases (TALENs), and RNA-guided nucleases, such as the CRISPR/Cas (clustered regularly interspaced short palindromic repeats/CRI...
Sakuma, Tetsushi; Ochiai, Hiroshi; Kaneko, Takehito; Mashimo, Tomoji; Tokumasu, Daisuke; Sakane, Yuto; Suzuki, Ken-ichi; Miyamoto, Tatsuo; Sakamoto, Naoaki; Matsuura, Shinya et al.
SCIENTIFIC REPORTS   3    Nov 2013   [Refereed]
Transcription activator-like effector (TALE) nuclease (TALEN) is a site-specific nuclease, which can be freely designed and easily constructed. Numerous methods of constructing TALENs harboring different TALE scaffolds and repeat variants have rec...
Kato, Tomoko; Miyata, Kohei; Sonobe, Miku; Yamashita, Satoshi; Tamano, Moe; Miura, Kento; Kanai, Yoshiakira; Miyamoto, Shingo; Sakuma, Tetsushi; Yamamoto, Takashi et al.
SCIENTIFIC REPORTS   3    Nov 2013   [Refereed]
Recently developed transcription activator-like effector nuclease (TALEN) technology has enabled the creation of knockout mice, even for genes on the Y chromosome. In this study, we generated a knockout mouse for Sry, a sex-determining gene on the...
Oulhen, Nathalie; Yoshida, Takaya; Yajima, Mamiko; Song, Jia L.; Sakuma, Tetsushi; Sakamoto, Naoaki; Yamamoto, Takashi; Wessel, Gary M.
DEVELOPMENTAL BIOLOGY   377(1) 275-283   May 2013   [Refereed]
Nanos is a translational regulator required for the survival and maintenance of primordial germ cells during embryogenesis. Three nanos homologs are present in the genome of the sea urchin Strongylocentrotus puipuratus (Sp), and each nanos mRNA ac...
Sakuma, Tetsushi; Hosoi, Sayaka; Woltjen, Knut; Suzuki, Ken-ichi; Kashiwagi, Keiko; Wada, Housei; Ochiai, Hiroshi; Miyamoto, Tatsuo; Kawai, Narudo; Sasakura, Yasunori et al.
GENES TO CELLS   18(4) 315-326   Apr 2013   [Refereed]
Transcription activatorlike effector nucleases (TALENs) have recently arisen as effective tools for targeted genome engineering. Here, we report streamlined methods for the construction and evaluation of TALENs based on the Golden Gate TALEN and T...
Hozumi, Akiko; Yoshida, Reiko; Horie, Takeo; Sakuma, Tetsushi; Yamamoto, Takashi; Sasakura, Yasunori
DEVELOPMENTAL BIOLOGY   375(1) 79-91   Mar 2013   [Refereed]
Enhancers are flexible in terms of their location and orientation relative to the genes they regulate. However, little is known about whether the flexibility can be applied in every combination of enhancers and genes. Enhancer detection with trans...
High efficiency TALENs enable F0 functional analysis by targeted gene disruption in Xenopus laevis embryos
Suzuki KT, Isoyama Y, Kashiwagi K, Sakuma T, Ochiai H, Sakamoto N, Furuno N, Kashiwagi A, Yamamoto T
Biol Open      Mar 2013
Efficient targeted mutagenesis in medaka using custom-designed transcription activator-like effector nucleases
Ansai S, Sakuma T, Yamamoto T, Ariga H, Uemura N, Takahashi R, Kinoshita M
Genetics      Mar 2013
Quantitative assay for TALEN activity at endogenous genomic loci
Hisano Y, Ota S, Arakawa K, Muraki M, Kono N, Oshita K, Sakuma T, Tomita M, Yamamoto T, Okada Y, Kawahara A
Biol Open      Feb 2013

Research Grants & Projects

 
Japan Agency for Medical Research and Development: Research Program on Hepatitis
Project Year: Apr 2018 - Mar 2019
Hospital, National Center for Geriatrics and Gerontology: Practical Research for Innovative Cancer Control
Project Year: Apr 2017 - Mar 2018
Hospital, National Center for Geriatrics and Gerontology: Practical Research for Innovative Cancer Control
Project Year: Apr 2017 - Mar 2018
Hospital, National Center for Geriatrics and Gerontology: Research Program on Hepatitis
Project Year: Apr 2017 - Mar 2018
Japan Agency for Medical Research and Development: Practical Research for Innovative Cancer Control
Project Year: Nov 2016 - Mar 2017