佐久間 哲史

J-GLOBALへ         更新日: 18/12/10 03:33
 
アバター
研究者氏名
佐久間 哲史
 
サクマ テツシ
ハンドル
Tetsushi Sakuma
eメール
tetsushi-sakumahiroshima-u.ac.jp
所属
広島大学
部署
数理分子生命理学専攻
職名
講師
学位
博士(理学)(広島大学), 修士(理学)(広島大学)
科研費研究者番号
90711143
ORCID ID
0000-0003-0396-1563

プロフィール

ゲノム編集の技術開発に携わりつつ、様々な細胞や生物での利用を推進しています。

研究分野

 
 

経歴

 
2012年4月
 - 
2013年3月
日本学術振興会 特別研究員PD
 
2013年4月
 - 
2015年3月
広島大学 大学院理学研究科 特任助教
 
2015年4月
 - 
2018年3月
広島大学 大学院理学研究科 特任講師
 
2018年4月
   
 
広島大学 大学院理学研究科 講師
 

学歴

 
2002年4月
 - 
2006年3月
大阪大学 歯学部 歯学科
 
2006年4月
 - 
2008年3月
広島大学 理学部 生物科学科
 
2008年4月
 - 
2010年3月
広島大学 大学院理学研究科 生物科学専攻
 
2010年4月
 - 
2012年3月
広島大学 大学院理学研究科 数理分子生命理学専攻
 

委員歴

 
2018年4月
 - 
2019年7月
文部科学省学術調査官  文部科学省研究振興局振興企画課
 
2018年8月
 - 
2020年6月
(社)日本ゲノム編集学会  教育実習委員
 
2018年8月
 - 
2020年6月
(社)日本ゲノム編集学会  幹事
 

受賞

 
2015年
広島大学 学長表彰
 
2016年
広島大学 Phoenix Outstanding Researcher Award 広島大学 Phoenix Outstanding Researcher Award
 
2017年
広島大学 Phoenix Outstanding Researcher Award 広島大学 Phoenix Outstanding Researcher Award
 

論文

 
Tanigawa, Shunsuke; Islam, Mazharul; Sharmin, Sazia; Naganuma, Hidekazu; Yoshimura, Yasuhiro; Haque, Fahim; Era, Takumi; Nakazato, Hitoshi; Nakanishi, Koichi; Sakuma, Tetsushi; Yamamoto, Takashi; Kurihara, Hidetake; Taguchi, Atsuhiro; Nishinakamura, Ryuichi
STEM CELL REPORTS   11(3) 727-740   2018年9月   [査読有り]
Mutations in the NPHS1 gene, which encodes NEPHRIN, cause congenital nephrotic syndrome, resulting from impaired slit diaphragm (SD) formation in glomerular podocytes. However, methods for SD reconstitution have been unavailable, thereby limiting ...
Nakade, Shota; Mochida, Keiji; Kunii, Atsushi; Nakamae, Kazuki; Aida, Tomomi; Tanaka, Kohichi; Sakamoto, Naoaki; Sakuma, Tetsushi; Yamamoto, Takashi
NATURE COMMUNICATIONS   9    2018年8月   [査読有り]
Selective genome editing such as gene knock-in has recently been achieved by administration of chemical enhancer or inhibitor of particular DNA double-strand break (DSB) repair pathways, as well as overexpression of pathway-specific genes. In this...
Kawabe, Yoshinori; Komatsu, Shinya; Komatsu, Shodai; Murakami, Mai; Ito, Akira; Sakuma, Tetsushi; Nakamura, Takahiro; Yamamoto, Takashi; Kamihira, Masamichi
JOURNAL OF BIOSCIENCE AND BIOENGINEERING   125(5) 599-605   2018年5月   [査読有り]
Chinese hamster ovary (CHO) cells have been used as host cells for the production of pharmaceutical proteins. For the high and stable production of target proteins, the transgene should be integrated into a suitable genomic locus of host cells. He...
Kim, Shin-Il; Matsumoto, Tomoko; Kagawa, Harunobu; Nakamura, Michiko; Hirohata, Ryoko; Ueno, Ayano; Ohishi, Maki; Sakuma, Tetsushi; Soga, Tomoyoshi; Yamamoto, Takashi; Woltjen, Knut
NATURE COMMUNICATIONS   9    2018年3月   [査読有り]
Gene-edited induced pluripotent stem cells (iPSCs) provide relevant isogenic human disease models in patient-specific or healthy genetic backgrounds. Towards this end, gene targeting using antibiotic selection along with engineered point mutations...
Saha, Liton Kumar; Kim, Sujin; Kang, Habyeong; Akter, Salma; Choi, Kyungho; Sakuma, Tetsushi; Yamamoto, Takashi; Sasanuma, Hiroyuki; Hirota, Kouji; Nakamura, Jun; Honma, Masamitsu; Takeda, Shunichi
ENVIRONMENTAL AND MOLECULAR MUTAGENESIS   59(6) 529-538   2018年7月   [査読有り]
The micronucleus (MN) test has become an attractive tool both for evaluating the genotoxicity of test chemicals because of its ability to detect clastogenic and aneugenic events and for its convenience. As the MN assay has been mostly performed us...
Sato'o, Yusuke; Hisatsune, Junzo; Yu, Liansheng; Sakuma, Tetsushi; Yamamoto, Takashi; Sugai, Motoyuki
PLOS ONE   13(1)    2018年1月   [査読有り]
Preparing the genetically modified organisms have required much time and labor, making it the rate-limiting step but CRISPR/Cas9 technology appearance has changed this difficulty. Although reports on CRISPR/Cas9 technology such as genome editing a...
Nakade, Shota; Yamamoto, Takashi; Sakuma, Tetsushi
JOURNAL OF HUMAN GENETICS   63(2) 187-194   2018年2月   [査読有り]
Cancer epigenetics is one of the most important research subjects in dissecting cancer mechanisms and therapeutic targets because the emergence and malignant transformation of various cancers are caused by unnatural expression of cancer-related ge...
Shukunami, Chisa; Takimoto, Aki; Nishizaki, Yuriko; Yoshimoto, Yuki; Tanaka, Seima; Miura, Shigenori; Watanabe, Hitomi; Sakuma, Tetsushi; Yamamoto, Takashi; Kondoh, Gen; Hiraki, Yuji
SCIENTIFIC REPORTS   8    2018年2月   [査読有り]
Tenomodulin (Tnmd) is a type II transmembrane glycoprotein predominantly expressed in tendons and ligaments. We found that scleraxis (Scx), a member of the Twist-family of basic helix-loop-helix transcription factors, is a transcriptional activato...
Takashina, Tomoki; Koyama, Takayoshi; Nohara, Satoshi; Hasegawa, Masakatsu; Ishiguro, Akira; Iijima, Kenta; Lu, Jun; Shimura, Mari; Okamura, Tadashi; Sakuma, Tetsushi; Yamamoto, Takashi; Ishizaka, Yukihito
BIOMATERIALS   173 11-21   2018年8月   [査読有り]
Cellular reprogramming is a promising technology in regenerative medicine, but most studies have been performed by using expression vectors. For future clinical applications, it is necessary to establish a system in which cell engineering can be m...
Sakuma, Tetsushi; Mochida, Keiji; Nakade, Shota; Ezure, Toru; Minagawa, Sachi; Yamamoto, Takashi
GENES TO CELLS   23(4) 255-263   2018年4月   [査読有り]
Single-cell cloning is an essential technique for establishing genome-edited cell clones mediated by programmable nucleases such as CRISPR-Cas9. However, residual genome-editing activity after single-cell cloning may cause heterogeneity in the clo...
Tsuda, Masataka; Cho, Kosai; Ooka, Masato; Shimizu, Naoto; Watanabe, Reiko; Yasui, Akira; Nakazawa, Yuka; Ogi, Tomoo; Harada, Hiroshi; Agama, Keli; Nakamura, Jun; Asada, Ryuta; Fujiike, Haruna; Sakuma, Tetsushi; Yamamoto, Takashi; Murai, Junko; Hiraoka, Masahiro; Koike, Kaoru; Pommier, Yves; Takeda, Shunichi; Hirota, Kouji
PLOS ONE   12(11)    2017年11月   [査読有り]
ALC1/CHD1L is a member of the SNF2 superfamily of ATPases carrying a macrodomain that binds poly(ADP-ribose). Poly(ADP-ribose) polymerase (PARP) 1 and 2 synthesize poly(ADP-ribose) at DNA-strand cleavage sites, promoting base excision repair (BER)...
Takayama, Kazuo; Igai, Keisuke; Hagihara, Yasuko; Hashimoto, Rina; Hanawa, Morifumi; Sakuma, Tetsushi; Tachibana, Masashi; Sakurai, Fuminori; Yamamoto, Takashi; Mizuguchi, Hiroyuki
NUCLEIC ACIDS RESEARCH   45(9) 5198-5207   2017年5月   [査読有り]
Genome editing research of human ES/iPS cells has been accelerated by clustered regularly interspaced short palindromic repeats/CRISPR-associated 9 (CRISPR/Cas9) and transcription activator-like effector nucleases (TALEN) technologies. However, th...
Nakagawa, Yoshiko; Sakuma, Tetsushi; Nishimichi, Norihisa; Yokosaki, Yasuyuki; Takeo, Toru; Nakagata, Naomi; Yamamoto, Takashi
BIOLOGY OPEN   6(5) 706-713   2017年5月   [査読有り]
Robust reproductive engineering techniques are required for the efficient and rapid production of genetically modified mice. We have reported the efficient production of genome-edited mice using reproductive engineering techniques, such as ultra-s...
Yoshida, Keita; Nakahata, Azusa; Treen, Nicholas; Sakuma, Tetsushi; Yamamoto, Takashi; Sasakura, Yasunori
DEVELOPMENT   144(9) 1629-1634   2017年5月   [査読有り]
The chordate pharynx, possessing gill slits and the endostyle, is a complex of multiple tissues that are highly organized along the anterior-posterior (AP) axis. Although Hox genes show AP coordinated expression in the pharyngeal endoderm, tissue-...
Mizutani, Osamu; Arazoe, Takayuki; Toshida, Kenji; Hayashi, Risa; Ohsato, Shuichi; Sakuma, Tetsushi; Yamamoto, Takashi; Kuwata, Shigeru; Yamada, Osamu
JOURNAL OF BIOSCIENCE AND BIOENGINEERING   123(3) 287-293   2017年3月   [査読有り]
Transcription activator-like effector nucleases (TALENs), which can generate DNA double-strand breaks at specific sites in the desired genome locus, have been used in many organisms as a tool for genome editing. In Aspergilli, including Aspergillu...
Yoshida, Keita; Hozumi, Akiko; Treen, Nicholas; Sakuma, Tetsushi; Yamamoto, Takashi; Shirae-Kurabayashi, Maki; Sasakura, Yasunori
DEVELOPMENTAL BIOLOGY   423(2) 111-125   2017年3月   [査読有り]
The ascidian Ciona intestinalis has a high regeneration capacity that enables the regeneration of artificially removed primordial germ cells (PGCs) from somatic cells. We utilized PGC regeneration to establish efficient methods of germ line mutage...
Kaku, Yusuke; Taguchi, Atsuhiro; Tanigawa, Shunsuke; Haque, Fahim; Sakuma, Tetsushi; Yamamoto, Takashi; Nishinakamura, Ryuichi
SCIENTIFIC REPORTS   7    2017年7月   [査読有り]
The kidney is formed by reciprocal interactions between the nephron progenitor and the ureteric bud, the former of which gives rise to the epithelia of nephrons consisting of glomeruli and renal tubules. The transcription factor PAX2 is essential ...
Nakade, Shota; Yamamoto, Takashi; Sakuma, Tetsushi
BIOENGINEERED   8(3) 265-273   2017年   [査読有り]
Since the rapid emergence of clustered regulatory interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein 9 (Cas9) system, developed as a genome engineering tool in 2012-2013, most researchers in the life science field have had a ...
Nakamae, Kazuki; Nishimura, Yuki; Takenaga, Mitsumasa; Nakade, Shota; Sakamoto, Naoaki; Ide, Hiroshi; Sakuma, Tetsushi; Yamamoto, Takashi
BIOENGINEERED   8(3) 302-308   2017年   [査読有り]
The emerging genome editing technology has enabled the creation of gene knock-in cells easily, efficiently, and rapidly, which has dramatically accelerated research in the field of mammalian functional genomics, including in humans. We recently de...
Nakamura, Minako; Kumrungsee, Thanutchaporn; Sakuma, Tetsushi; Yamamoto, Takashi; Yanaka, Noriyuki
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   81(11) 2164-2167   2017年   [査読有り]
In this study, we investigated the physiological function of glycerophosphodiesterase 5 (GDE5) in the proliferation of NIH3T3 fibroblasts. We used transcription activator-like effector nuclease (TALEN) in NIH3T3 cells with an intron targeting-medi...
Aida, Tomomi; Nakade, Shota; Sakuma, Tetsushi; Izu, Yayoi; Oishi, Ayu; Mochida, Keiji; Ishikubo, Harumi; Usami, Takako; Aizawa, Hidenori; Yamamoto, Takashi; Tanaka, Kohichi
BMC GENOMICS   17    2016年11月   [査読有り]
Background: Although CRISPR/Cas enables one-step gene cassette knock-in, assembling targeting vectors containing long homology arms is a laborious process for high-throughput knock-in. We recently developed the CRISPR/Cas-based precise integration...
Tochio, Naoya; Umehara, Kohei; Uewaki, Jun-ichi; Flechsig, Holger; Kondo, Masaharu; Dewa, Takehisa; Sakuma, Tetsushi; Yamamoto, Takashi; Saitoh, Takashi; Togashi, Yuichi; Tate, Shin-ichi
SCIENTIFIC REPORTS   6    2016年11月   [査読有り]
Transcription activator-like effector (TALE) nuclease (TALEN) is widely used as a tool in genome editing. The DNA binding part of TALEN consists of a tandem array of TAL-repeats that form a right-handed superhelix. Each TAL-repeat recognises a spe...
Sakuma, Tetsushi; Masaki, Keiichi; Abe-Chayama, Hiromi; Mochida, Keiji; Yamamoto, Takashi; Chayama, Kazuaki
GENES TO CELLS   21(11) 1253-1262   2016年11月   [査読有り]
CRISPR-Cas9-mediated genome-editing technology contributes not only to basic genomic studies but also to clinical studies such as genetic correction and virus inactivation. Hepatitis B virus (HBV) is a major target for potential application of CRI...
Marsan, Elise; Ishida, Saeko; Schramm, Adrien; Weckhuysen, Sarah; Muraca, Giuseppe; Lecas, Sarah; Liang, Ning; Treins, Caroline; Pende, Mario; Roussel, Delphine; Le Van Quyen, Michel; Mashimo, Tomoji; Kaneko, Takehito; Yamamoto, Takashi; Sakuma, Tetsushi; Mahon, Severine; Miles, Richard; Leguern, Eric; Charpier, Stephane; Baulac, Stephanie
NEUROBIOLOGY OF DISEASE   89 180-189   2016年5月   [査読有り]
DEP-domain containing 5 (DEPDC5), encoding a repressor of the mechanistic target of rapamycin complex 1 (mTORC1) signaling pathway, has recently emerged as a major gene mutated in familial focal epilepsies and focal cortical dysplasia. Here we est...
Nakahara, Yoshinari; Muto, Akihiko; Hirabayashi, Ryo; Sakuma, Tetsushi; Yamamoto, Takashi; Kume, Shoen; Kikuchi, Yutaka
GENES TO CELLS   21(5) 492-504   2016年5月   [査読有り]
The adenohypophysis (AH) consists of six distinct types of hormone-secreting cells. In zebrafish, although proper differentiation of all AH cell types has been shown to require Notch signaling within a period of 14-16h postfertilization (hpf), the...
Nii, Takenobu; Kohara, Hiroshi; Marumoto, Tomotoshi; Sakuma, Tetsushi; Yamamoto, Takashi; Tani, Kenzaburo
BIORESEARCH OPEN ACCESS   5(1) 127-136   2016年5月   [査読有り]
Efficient gene transfer into human pluripotent stem cells (hPSCs) holds great promise for regenerative medicine and pharmaceutical development. In the past decade, various methods were developed for gene transfer into hPSCs; however, hPSCs form ti...
Banno, Kimihiko; Omori, Sayaka; Hirata, Katsuya; Nawa, Nobutoshi; Nakagawa, Natsuki; Nishimura, Ken; Ohtaka, Manami; Nakanishi, Mahito; Sakuma, Tetsushi; Yamamoto, Takashi; Toki, Tsutomu; Ito, Etsuro; Yamamoto, Toshiyuki; Kokubu, Chikara; Takeda, Junji; Taniguchi, Hidetoshi; Arahori, Hitomi; Wada, Kazuko; Kitabatake, Yasuji; Ozono, Keiichi
CELL REPORTS   15(6) 1228-1241   2016年5月   [査読有り]
Chromosomal aneuploidy and specific gene mutations are recognized early hallmarks of many oncogenic processes. However, the net effect of these abnormalities has generally not been explored. We focused on transient myeloproliferative disorder (TMD...
Sharmin, Sazia; Taguchi, Atsuhiro; Kaku, Yusuke; Yoshimura, Yasuhiro; Ohmori, Tomoko; Sakuma, Tetsushi; Mukoyama, Masashi; Yamamoto, Takashi; Kurihara, Hidetake; Nishinakamura, Ryuichi
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY   27(6) 1778-1791   2016年6月   [査読有り]
Glomerular podocytes express proteins, such as nephrin, that constitute the slit diaphragm, thereby contributing to the filtration process in the kidney. Glomerular development has been analyzed mainly in mice, whereas analysis of human kidney dev...
Takata, Nozomu; Sakakura, Eriko; Kasukawa, Takeya; Sakuma, Tetsushi; Yamamoto, Takashi; Sasai, Yoshiki
HUMAN GENE THERAPY   27(6) 436-450   2016年6月   [査読有り]
The epiblast (foremost embryonic ectoderm) generates all three germ layers and therefore has crucial roles in the formation of all mammalian body cells. However, regulation of epiblast gene expression is poorly understood because of the difficulty...
Generation of a Nonhuman Primate Model of Severe Combined Immunodeficiency Using Highly Efficient Genome Editing
Sato, Kenya; Oiwa, Ryo; Kumita, Wakako; Henry, Rachel; Sakuma, Tetsushi; Ito, Ryoji; Nozu, Ryoko; Inoue, Takashi; Katano, Ikumi; Sato, Kengo; Okahara, Norio; Okahara, Junko; Shimizu, Yoshihisa; Yamamoto, Masafumi; Hanazawa, Kisaburo; Kawakami, Takao; Kametani, Yoshie; Suzuki, Ryuji; Takahashi, Takeshi; Weinstein, Edward J.; Yamamoto, Takashi; Sakakibara, Yasubumi; Habu, Sonoko; Hata, Jun-ichi; Okano, Hideyuki; Sasaki, Erika
CELL STEM CELL   19(1) 127-138   2016年7月   [査読有り]
Recent advances in genome editing have facilitated the generation of nonhuman primate (NHP) models, with potential to unmask the complex biology of human disease not revealed by rodent models. However, their broader use is hindered by the challeng...
Involvement of aspartoacylase in tremor expression in rats
Nishitani, Ai; Tanaka, Miyuu; Shimizu, Saki; Kunisawa, Naofumi; Yokoe, Mayuko; Yoshida, Yusaku; Suzuki, Toshiro; Sakuma, Tetsushi; Yamamoto, Takashi; Kuwamura, Mitsuru; Takenaka, Shigeo; Ohno, Yukihiro; Kuramoto, Takashi
EXPERIMENTAL ANIMALS   65(3) 293-301   2016年7月   [査読有り]
Essential tremor (ET) is a common movement disorder with a poorly understood etiology. The TRM/Kyo mutant rat, showing spontaneous tremor, is an animal model of ET. Recently, we demonstrated that tremors in these rats emerge when two mutant loci, ...
Guo, Long; Yamashita, Hiroshi; Kou, Ikuyo; Takimoto, Aki; Meguro-Horike, Makiko; Horike, Shin-ichi; Sakuma, Tetsushi; Miura, Shigenori; Adachi, Taiji; Yamamoto, Takashi; Ikegawa, Shiro; Hiraki, Yuji; Shukunami, Chisa
PLOS GENETICS   12(1)    2016年1月   [査読有り]
Previously, we identified an adolescent idiopathic scoliosis susceptibility locus near human ladybird homeobox 1 (LBX1) and FLJ41350 by a genome-wide association study. Here, we characterized the associated non-coding variant and investigated the ...
Rao, Shengbin; Fujimura, Tatsuya; Matsunari, Hitomi; Sakuma, Tetsushi; Nakano, Kazuaki; Watanabe, Masahito; Asano, Yoshinori; Kitagawa, Eri; Yamamoto, Takashi; Nagashima, Hiroshi
MOLECULAR REPRODUCTION AND DEVELOPMENT   83(1) 61-70   2016年1月   [査読有り]
Myostatin (MSTN) is a negative regulator of myogenesis, and disruption of its function causes increased muscle mass in various species. Here, we report the generation of MSTN-knockout (KO) pigs using genome editing technology combined with somatic...
Sakuma, Tetsushi; Nakade, Shota; Sakane, Yuto; Suzuki, Ken-Ichi T; Yamamoto, Takashi
NATURE PROTOCOLS   11(1) 118-133   2016年1月   [査読有り]
Programmable nucleases enable engineering of the genome by utilizing endogenous DNA double-strand break (DSB) repair pathways. Although homologous recombination (HR)-mediated gene knock-in is well established, it cannot necessarily be applied in e...
Sasakura, Yasunori; Ogura, Yosuke; Treen, Nicholas; Yokomori, Rui; Park, Sung-Joon; Nakai, Kenta; Saiga, Hidetoshi; Sakuma, Tetsushi; Yamamoto, Takashi; Fujiwara, Shigeki; Yoshida, Keita
PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES   283(1845)    2016年12月   [査読有り]
The horizontal transfer of genes between distantly related organisms is undoubtedly a major factor in the evolution of novel traits. Because genes are functionless without expression, horizontally transferred genes must acquire appropriate transcr...
Sasaki, Takako; Hanisch, Franz-Georg; Deutzmann, Rainer; Sakai, Lynn Y.; Sakuma, Tetsushi; Miyamoto, Tatsuo; Yamamoto, Takashi; Hannappel, Ewald; Chu, Mon-Li; Lanig, Harald; von der Mark, Klaus
MATRIX BIOLOGY   56 132-149   2016年12月   [査読有り]
Fibulin-4 is a 60 kDa calcium binding glycoprotein that has an important role in development and integrity of extracellular matrices. It interacts with elastin, fibrillin-1 and collagen IV as well as with lysyl oxidases and is involved in elastoge...
Toyonaga, Kenji; Torigoe, Shota; Motomura, Yoshitomo; Kamichi, Takane; Hayashi, Jennifer M.; Morita, Yasu S.; Noguchi, Naoto; Chuma, Yasushi; Kiyohara, Hideyasu; Matsuo, Kazuhiro; Tanaka, Hiroshi; Nakagawa, Yoshiko; Sakuma, Tetsushi; Ohmuraya, Masaki; Yamamoto, Takashi; Umemura, Masayuki; Matsuzaki, Goro; Yoshikai, Yasunobu; Yano, Ikuya; Miyamoto, Tomofumi; Yamasaki, Sho
IMMUNITY   45(6) 1245-1257   2016年12月   [査読有り]
Phosphatidyl-inositol mannosides (PIM) are glycolipids unique to mycobacteria and other related bacteria that stimulate host immune responses and are implicated in mycobacteria pathogenicity. Here, we found that the FcRg-coupled C-type lectin rece...
Nakagawa, Yoshiko; Sakuma, Tetsushi; Nishimichi, Norihisa; Yokosaki, Yasuyuki; Yanaka, Noriyuki; Takeo, Toru; Nakagata, Naomi; Yamamoto, Takashi
BIOLOGY OPEN   5(8) 1142-1148   2016年8月   [査読有り]
Current advances in producing genetically modified mice using genome-editing technologies have indicated the need for improvement of limiting factors including zygote collection for microinjection and their cryopreservation. Recently, we developed...
Suzuki, Miyuki; Takagi, Chiyo; Miura, Shinichirou; Sakane, Yuto; Suzuki, Makoto; Sakuma, Tetsushi; Sakamoto, Naoaki; Endo, Tetsuya; Kamei, Yasuhiro; Sato, Yuko; Kimura, Hiroshi; Yamamoto, Takashi; Ueno, Naoto; Suzuki, Ken-ichi T.
GENES TO CELLS   21(4) 358-369   2016年4月   [査読有り]
Xenopus laevis tadpoles can completely regenerate their appendages, such as tail and limbs, and therefore provide a unique model to decipher the molecular mechanisms of organ regeneration in vertebrates. Epigenetic modifications are likely to be i...
Ichiyanagi, Naoki; Fujimori, Koki; Yano, Masato; Ishihara-Fujisaki, Chikako; Sone, Takefumi; Akiyama, Tetsuya; Okada, Yohei; Akamatsu, Wado; Matsumoto, Takuya; Ishikawa, Mitsuru; Nishimoto, Yoshinori; Ishihara, Yasuharu; Sakuma, Tetsushi; Yamamoto, Takashi; Tsuiji, Hitomi; Suzuki, Naoki; Warita, Hitoshi; Aoki, Masashi; Okano, Hideyuki
STEM CELL REPORTS   6(4) 496-510   2016年4月   [査読有り]
Amyotrophic lateral sclerosis (ALS) is a late-onset motor neuron disorder. Although its neuropathology is well understood, the cellular and molecular mechanisms are yet to be elucidated due to limitations in the currently available human genetic d...
Sakuma, Tetsushi; Takenaga, Mitsumasa; Kawabe, Yoshinori; Nakamura, Takahiro; Kamihira, Masamichi; Yamamoto, Takashi
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES   16(10) 23849-23866   2015年10月   [査読有り]
Gene knock-in techniques have rapidly evolved in recent years, along with the development and maturation of genome editing technology using programmable nucleases. We recently reported a novel strategy for microhomology-mediated end-joining-depend...
Nakade, Shota; Sakuma, Tetsushi; Sakane, Yuto; Hara, Yoshihiro; Kurabayashi, Atsushi; Kashiwagi, Keiko; Kashiwagi, Akihiko; Yamamoto, Takashi; Obara, Masanobu
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL   51(9) 879-884   2015年10月   [査読有り]
Transcription activator-like effector nucleases (TALENs) have previously been used for targeted genome editing in various organisms including Xenopus laevis. However, because of genomic polyploidization, X. laevis usually possess homeologous genes...
Fujiwara, Miwako; Nagatomo, Azusa; Tsuda, Megumi; Obata, Shuichi; Sakuma, Tetsushi; Yamamoto, Takashi; Suzuki, Shintaro T.
JOURNAL OF BIOCHEMISTRY   158(4) 339-353   2015年10月   [査読有り]
The role of the juxtamembrane region of the desmocollin-2 cytoplasmic domain in desmosome formation was investigated by using gene knockout and reconstitution experiments. When a deletion construct of the desmocollin-2 juxtamembrane region was exp...
Miyamoto, Kei; Suzuki, Ken-ichi T.; Suzuki, Miyuki; Sakane, Yuto; Sakuma, Tetsushi; Herberg, Sarah; Simeone, Angela; Simpson, David; Jullien, Jerome; Yamamoto, Takashi; Gurdon, J. B.
PLOS ONE   10(11)    2015年11月   [査読有り]
Recent advances in genome editing using programmable nucleases have revolutionized gene targeting in various organisms. Successful gene knock-out has been shown in Xenopus, a widely used model organism, although a system enabling less mosaic knock...
Nakagawa, Yoshiko; Sakuma, Tetsushi; Sakamoto, Takuya; Ohmuraya, Masaki; Nakagata, Naomi; Yamamoto, Takashi
BMC BIOTECHNOLOGY   15    2015年5月   [査読有り]
Background: Clustered regulatory interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9)-mediated genome editing permits the rapid production of genetically engineered mice. To make the most of this innovative technology,...
Hisano, Yu; Sakuma, Tetsushi; Nakade, Shota; Ohga, Rie; Ota, Satoshi; Okamoto, Hitoshi; Yamamoto, Takashi; Kawahara, Atsuo
SCIENTIFIC REPORTS   5    2015年3月   [査読有り]
The CRISPR/Cas9 system provides a powerful tool for genome editing in various model organisms, including zebrafish. The establishment of targeted gene-disrupted zebrafish (knockouts) is readily achieved by CRISPR/Cas9-mediated genome modification....
Ebina, Hirotaka; Kanemura, Yuka; Misawa, Naoko; Sakuma, Tetsushi; Kobayashi, Tomoko; Yamamoto, Takashi; Koyanagi, Yoshio
PLOS ONE   10(3)    2015年3月   [査読有り]
DNA-editing technology has made it possible to rewrite genetic information in living cells. Human immunodeficiency virus (HIV) provirus, an integrated form of viral complementary DNA in host chromosomes, could be a potential target for this techno...
Hara, Satoshi; Tamano, Moe; Yamashita, Satoshi; Kato, Tomoko; Saito, Takeshi; Sakuma, Tetsushi; Yamamoto, Takashi; Inui, Masafumi; Takada, Shuji
SCIENTIFIC REPORTS   5    2015年6月   [査読有り]
Genome editing, which introduces mutations in genes of interest using artificial DNA nucleases such as the ZFN, TALEN, and CRISPR/Cas9 systems in living cells, is a useful tool for generating mutant animals. Although CRISPR/Cas9 provides advantage...
Keka, Islam Shamima; Mohiuddin; Maede, Yuko; Rahman, Md Maminur; Sakuma, Tetsushi; Honma, Masamitsu; Yamamoto, Takashi; Takeda, Shunichi; Sasanuma, Hiroyuki
NUCLEIC ACIDS RESEARCH   43(13) 6359-6372   2015年7月   [査読有り]
Smarcal1 is a SWI/SNF-family protein with an ATPase domain involved in DNA-annealing activities and a binding site for the RPA single-strand-DNA-binding protein. Although the role played by Smarcal1 in the maintenance of replication forks has been...
Arazoe, Takayuki; Ogawa, Tetsuo; Miyoshi, Kennosuke; Yamato, Tohru; Ohsato, Shuichi; Sakuma, Tetsushi; Yamamoto, Takashi; Arie, Tsutomu; Kuwata, Shigeru
BIOTECHNOLOGY AND BIOENGINEERING   112(7) 1335-1342   2015年7月   [査読有り]
Genetic manipulation is key to unraveling gene functions and creating genetically modified strains of microbial organisms. Recently, engineered nucleases that can generate DNA double-strand breaks (DSBs) at a specific site in the desired locus wit...
Sato, Takuya; Sakuma, Tetsushi; Yokonishi, Tetsuhiro; Katagiri, Kumiko; Kamimura, Satoshi; Ogonuki, Narumi; Ogura, Atsuo; Yamamoto, Takashi; Ogawa, Takehiko
STEM CELL REPORTS   5(1) 75-82   2015年7月   [査読有り]
Mouse spermatogonial stem cells (SSCs) can be cultured for multiplication and maintained for long periods while preserving their spermatogenic ability. Although the cultured SSCs, named germline stem (GS) cells, are targets of genome modification,...
Kawai, Narudo; Ogura, Yosuke; Ikuta, Tetsuro; Saiga, Hidetoshi; Hamada, Mayuko; Sakuma, Tetsushi; Yamamoto, Takashi; Satoh, Nori; Sasakura, Yasunori
DEVELOPMENTAL BIOLOGY   403(1) 43-56   2015年7月   [査読有り]
Hox cluster genes play crucial roles in development of the metazoan antero-posterior axis. Functions of Hox genes in patterning the central nervous system and limb buds are well known. They are also expressed in chordate endodermal tissues, where ...
Li, Hongmei Lisa; Fujimoto, Naoko; Sasakawa, Noriko; Shirai, Saya; Ohkame, Tokiko; Sakuma, Tetsushi; Tanaka, Michihiro; Amano, Naoki; Watanabe, Akira; Sakurai, Hidetoshi et al.
STEM CELL REPORTS   4(1) 143-154   2015年1月   [査読有り]
Duchenne muscular dystrophy (DMD) is a severe muscle-degenerative disease caused by a mutation in the dystrophin gene. Genetic correction of patient-derived induced pluripotent stem cells (iPSCs) by TALENs or CRISPR-Cas9 holds promise for DMD gene...
Choi, Jinyoung; Suzuki, Ken-ichi T.; Sakuma, Tetsushi; Shewade, Leena; Yamamoto, Takashi; Buchholz, Daniel R.
ENDOCRINOLOGY   156(2) 735-744   2015年2月   [査読有り]
Thyroid hormone (TH) receptor (TR) expression begins early in development in all vertebrates when circulating TH levels are absent or minimal, yet few developmental roles for unliganded TRs have been established. Unliganded TRs are expected to rep...
Miyamoto, Tatsuo; Hosoba, Kosuke; Ochiai, Hiroshi; Royba, Ekaterina; Izumi, Hideki; Sakuma, Tetsushi; Yamamoto, Takashi; Dynlacht, Brian David; Matsuura, Shinya
CELL REPORTS   10(5) 664-673   2015年2月   [査読有り]
The primary cilium is an antenna-like, microtubule-based organelle on the surface of most vertebrate cells for receiving extracellular information. Although primary cilia form in the quiescent phase, ciliary disassembly occurs when quiescent cells...
Nguyen Ngoc Hoa; Akagawa, Remi; Yamasaki, Tomomi; Hirota, Kouji; Sasa, Kentaro; Natsume, Toyoaki; Kobayashi, Junya; Sakuma, Tetsushi; Yamamoto, Takashi; Komatsu, Kenshi; Kanemaki, Masato T.; Pommier, Yves; Takeda, Shunichi; Sasanuma, Hiroyuki
GENES TO CELLS   20(12) 1059-1076   2015年12月   [査読有り]
Homologous recombination (HR) is initiated by double-strand break (DSB) resection, during which DSBs are processed by nucleases to generate 30 single-strand DNA. DSB resection is initiated by CtIP and Mre11 followed by long-range resection by Dna2...
Sasaki, Kotaro; Yokobayashi, Shihori; Nakamura, Tomonori; Okamoto, Ikuhiro; Yabuta, Yukihiro; Kurimoto, Kazuki; Ohta, Hiroshi; Moritoki, Yoshinobu; Iwatani, Chizuru; Tsuchiya, Hideaki; Nakamura, Shinichiro; Sekiguchi, Kiyotoshi; Sakuma, Tetsushi; Yamamoto&
CELL STEM CELL   17(2) 178-194   2015年8月   [査読有り]
Mechanisms underlying human germ cell development are unclear, partly due to difficulties in studying human embryos and lack of suitable experimental systems. Here, we show that human induced pluripotent stem cells (hiPSCs) differentiate into inci...
Aida, Tomomi; Chiyo, Keiho; Usami, Takako; Ishikubo, Harumi; Imahashi, Risa; Wada, Yusaku; Tanaka, Kenji F.; Sakuma, Tetsushi; Yamamoto, Takashi; Tanaka, Kohichi
GENOME BIOLOGY   16    2015年4月   [査読有り]
Although the CRISPR/Cas system has enabled one-step generation of knockout mice, low success rates of cassette knock-in limit its application range. Here we show that cloning-free, direct nuclear delivery of Cas9 protein complex with chemically sy...
Epithelial DLD-1 Cells with Disrupted E-cadherin Gene Retain the Ability to Form Cell Junctions and Apico-basal Polarity
Fujiwara, Miwako; Fujimura, Kihito; Obata, Shuichi; Yanagibashi, Ryo; Sakuma, Tetsushi; Yamamoto, Takashi; Suzuki, Shintaro T.
CELL STRUCTURE AND FUNCTION   40(2) 79-94   2015年   [査読有り]
Gene editing methods were applied to the study of E-cadherin function in epithelial cells. The Ecadherin gene in epithelial DLD-1 cells was ablated using TALEN. The resultant cells showed round fibroblastlike morphology and had almost no Ca2+-depe...
Sawai, Satoru; Ohyama, Kiyoshi; Yasumoto, Shuhei; Seki, Hikaru; Sakuma, Tetsushi; Yamamoto, Takashi; Takebayashi, Yumiko; Kojima, Mikiko; Sakakibara, Hitoshi; Aoki, Toshio et al.
PLANT CELL   26(9) 3763-3774   2014年9月   [査読有り]
Potatoes (Solanum tuberosum) contain alpha-solanine and alpha-chaconine, two well-known toxic steroidal glycoalkaloids (SGAs). Sprouts and green tubers accumulate especially high levels of SGAs. Although SGAs were proposed to be biosynthesized fro...
Kaneko, Takehito; Sakuma, Tetsushi; Yamamoto, Takashi; Mashimo, Tomoji
SCIENTIFIC REPORTS   4    2014年10月   [査読有り]
Engineered endonucleases, such as zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated (Cas) system, provide a powerful ...
Nakade, Shota; Tsubota, Takuya; Sakane, Yuto; Kume, Satoshi; Sakamoto, Naoaki; Obara, Masanobu; Daimon, Takaaki; Sezutsu, Hideki; Yamamoto, Takashi; Sakuma, Tetsushi et al.
NATURE COMMUNICATIONS   5    2014年11月   [査読有り]
Genome engineering using programmable nucleases enables homologous recombination (HR)-mediated gene knock-in. However, the labour used to construct targeting vectors containing homology arms and difficulties in inducing HR in some cell type and or...
Ochiai, Hiroshi; Sugawara, Takeshi; Sakuma, Tetsushi; Yamamoto, Takashi
SCIENTIFIC REPORTS   4    2014年11月   [査読有り]
Mouse embryonic stem cells (mESCs) are self-renewing and capable of differentiating into any of the three germ layers. An interesting feature of mESCs is the presence of cell-to-cell heterogeneity in gene expression that may be responsible for cel...
Hiruta, Chizue; Ogino, Yukiko; Sakuma, Tetsushi; Toyota, Kenji; Miyagawa, Shinichi; Yamamoto, Takashi; Iguchi, Taisen
BMC BIOTECHNOLOGY   14    2014年11月   [査読有り]
Background: The cosmopolitan microcrustacean Daphnia pulex provides a model system for both human health research and monitoring ecosystem integrity. It is the first crustacean to have its complete genome sequenced, an unprecedented ca. 36% of whi...
Yoshida, Keita; Treen, Nicholas; Hozumi, Akiko; Sakuma, Tetsushi; Yamamoto, Takashi; Sasakura, Yasunori
GENESIS   52(5) 431-439   2014年5月   [査読有り]
Targeted mutagenesis of genes-of-interest, or gene-knockout, is a powerful method to address the functions of genes. Engineered nucleases have enabled this approach in various organisms because of their ease of use. The ascidian Ciona intestinalis...
Tokumasu, Daisuke; Sakuma, Tetsushi; Hayashi, Yoko; Hosoi, Sayaka; Hiyama, Eiso; Yamamoto, Takashi
GENES TO CELLS   19(5) 419-431   2014年5月   [査読有り]
Transcription activator-like effector nuclease (TALEN)-mediated genome editing is a powerful technique for analyzing gene functions in various cells and organisms. At target loci, TALENs can not only introduce short insertions and deletions, but a...
Sakuma, Tetsushi; Nishikawa, Ayami; Kume, Satoshi; Chayama, Kazuaki; Yamamoto, Takashi
SCIENTIFIC REPORTS   4    2014年6月   [査読有り]
CRISPR/Cas9-mediated genome editing is a next-generation strategy for genetic modifications, not only for single gene targeting, but also for multiple targeted mutagenesis. To make the most of the multiplexity of CRISPR/Cas9, we established a syst...
Application of Oocyte Cryopreservation Technology in TALEN-Mediated Mouse Genome Editing
Nakagawa, Yoshiko; Sakuma, Tetsushi; Nakagata, Naomi; Yamasaki, Sho; Takeda, Naoki; Ohmuraya, Masaki; Yamamoto, Takashi
EXPERIMENTAL ANIMALS   63(3) 349-355   2014年7月   [査読有り]
Reproductive engineering techniques, such as in vitro fertilization (IVF) and cryopreservation of embryos or spermatozoa, are essential for preservation, reproduction, and transportation of genetically engineered mice. However, it has not yet been...
Yasue, Akihiro; Mitsui, Silvia Naomi; Watanabe, Takahito; Sakuma, Tetsushi; Oyadomari, Seiichi; Yamamoto, Takashi; Noji, Sumihare; Mito, Taro; Tanaka, Eiji
SCIENTIFIC REPORTS   4    2014年7月   [査読有り]
Since the establishment of embryonic stem (ES) cell lines, the combined use of gene targeting with homologous recombination has aided in elucidating the functions of various genes. However, the ES cell technique is inefficient and time-consuming. ...
Ochiai, Hiroshi; Miyamoto, Tatsuo; Kanai, Akinori; Hosoba, Kosuke; Sakuma, Tetsushi; Kudo, Yoshiki; Asami, Keiko; Ogawa, Atsushi; Watanabe, Akihiro; Kajii, Tadashi et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   111(4) 1461-1466   2014年1月   [査読有り]
Cancer-prone syndrome of premature chromatid separation with mosaic variegated aneuploidy [PCS (MVA) syndrome] is a rare autosomal recessive disorder characterized by constitutional aneuploidy and a high risk of childhood cancer. We previously rep...
Sugi, Takuma; Sakuma, Tetsushi; Ohtani, Yasuko; Yamamoto, Takashi
DEVELOPMENT GROWTH & DIFFERENTIATION   56(1) 78-85   2014年1月   [査読有り]
Targeted genome editing using transcription activator-like effector nuclease (TALEN) and clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 systems has recently emerged as a potentially powerful method for creating locus-speci...
Sakane, Yuto; Sakuma, Tetsushi; Kashiwagi, Keiko; Kashiwagi, Akihiko; Yamamoto, Takashi; Suzuki, Ken-ichi T.
DEVELOPMENT GROWTH & DIFFERENTIATION   56(1) 108-114   2014年1月   [査読有り]
Transcription activator-like effector nucleases (TALENs) have been extensively used in genome editing in various organisms. In some cases, however, it is difficult to efficiently disrupt both paralogous genes using a single pair of TALENs in Xenop...
Hosoi, Sayaka; Sakuma, Tetsushi; Sakamoto, Naoaki; Yamamoto, Takashi
DEVELOPMENT GROWTH & DIFFERENTIATION   56(1) 92-97   2014年1月   [査読有り]
Genome editing with engineered nucleases such as zinc-finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs) has been reported in various animals. We previously described ZFN-mediated targeted mutagenesis and insertio...
Hayashi, Toshinori; Sakamoto, Kousuke; Sakuma, Tetsushi; Yokotani, Naoki; Inoue, Takeshi; Kawaguchi, Eri; Agata, Kiyokazu; Yamamoto, Takashi; Takeuchi, Takashi
DEVELOPMENT GROWTH & DIFFERENTIATION   56(1) 115-121   2014年1月   [査読有り]
Regeneration of a lost tissue in an animal is an important issue. Although regenerative studies have a history of research spanning more than a century, the gene functions underlying regulation of the regeneration are mostly unclear. Analysis of k...
Kondo, Takefumi; Sakuma, Tetsushi; Wada, Housei; Akimoto-Kato, Ai; Yamamoto, Takashi; Hayashi, Shigeo
DEVELOPMENT GROWTH & DIFFERENTIATION   56(1) 86-91   2014年1月   [査読有り]
We report here a case study of TALEN-induced gene knock out of the trachealess gene of Drosophila. Two pairs of TALEN constructs caused targeted mutation in the germ line of 39% and 17% of injected animals, respectively. In the extreme case 100% o...
Sakuma, Tetsushi; Woltjen, Knut
DEVELOPMENT GROWTH & DIFFERENTIATION   56(1) 2-13   2014年1月   [査読有り]
Genome editing with engineered endonucleases is rapidly becoming a staple method in developmental biology studies. Engineered nucleases permit random or designed genomic modification at precise loci through the stimulation of endogenous double-str...
Screening Methods to Identify TALEN-Mediated Knockout Mice
Nakagawa, Yoshiko; Yamamoto, Takashi; Suzuki, Ken-Ichi; Araki, Kimi; Takeda, Naoki; Ohmuraya, Masaki; Sakuma, Tetsushi
EXPERIMENTAL ANIMALS   63(1) 79-84   2014年1月   [査読有り]
Genome editing with site-specific nucleases, such as zinc-finger nucleases or transcription activator-like effector nucleases (TALENs), and RNA-guided nucleases, such as the CRISPR/Cas (clustered regularly interspaced short palindromic repeats/CRI...
Treen, Nicholas; Yoshida, Keita; Sakuma, Tetsushi; Sasaki, Haruka; Kawai, Narudo; Yamamoto, Takashi; Sasakura, Yasunori
DEVELOPMENT   141(2) 481-487   2014年1月   [査読有り]
Custom designed nucleases can simplify gene targeting experiments and have the potential to allow these techniques to be performed in a wide range of organisms. Transcriptional activator-like effector nucleases (TALENs) are starting to fulfill thi...
Ninagawa, Satoshi; Okada, Tetsuya; Sumitomo, Yoshiki; Kamiya, Yukiko; Kato, Koichi; Horimoto, Satoshi; Ishikawa, Tokiro; Takeda, Shunichi; Sakuma, Tetsushi; Yamamoto, Takashi et al.
JOURNAL OF CELL BIOLOGY   206(3) 347-356   2014年8月   [査読有り]
Glycoproteins misfolded in the endoplasmic reticulum (ER) are subjected to ER-associated glycoprotein degradation (gpERAD) in which Htm1 -mediated mannose trimming from the oligosaccharide Man(8)GIcNAc(2) to Man(7)GIcNAc(2) is the rate-limiting st...
Kazuki, Yasuhiro; Yakura, Yuwna; Abe, Satoshi; Osaki, Mitsuhiko; Kajitani, Naoyo; Kazuki, Kanako; Takehara, Shoko; Honma, Kazuhisa; Suemori, Hirofumi; Yamazaki, Satoshi et al.
SCIENTIFIC REPORTS   4    2014年8月   [査読有り]
Infants with Down syndrome (DS) are at a high risk of developing transient abnormal myelopoiesis (TAM). A GATA1 mutation leading to the production of N-terminally truncated GATA1 (GATA1s) in early megakaryocyte/erythroid progenitors is linked to t...
Kato, Tomoko; Miyata, Kohei; Sonobe, Miku; Yamashita, Satoshi; Tamano, Moe; Miura, Kento; Kanai, Yoshiakira; Miyamoto, Shingo; Sakuma, Tetsushi; Yamamoto, Takashi et al.
SCIENTIFIC REPORTS   3    2013年11月   [査読有り]
Recently developed transcription activator-like effector nuclease (TALEN) technology has enabled the creation of knockout mice, even for genes on the Y chromosome. In this study, we generated a knockout mouse for Sry, a sex-determining gene on the...
Sakuma, Tetsushi; Ochiai, Hiroshi; Kaneko, Takehito; Mashimo, Tomoji; Tokumasu, Daisuke; Sakane, Yuto; Suzuki, Ken-ichi; Miyamoto, Tatsuo; Sakamoto, Naoaki; Matsuura, Shinya et al.
SCIENTIFIC REPORTS   3    2013年11月   [査読有り]
Transcription activator-like effector (TALE) nuclease (TALEN) is a site-specific nuclease, which can be freely designed and easily constructed. Numerous methods of constructing TALENs harboring different TALE scaffolds and repeat variants have rec...
Oulhen, Nathalie; Yoshida, Takaya; Yajima, Mamiko; Song, Jia L.; Sakuma, Tetsushi; Sakamoto, Naoaki; Yamamoto, Takashi; Wessel, Gary M.
DEVELOPMENTAL BIOLOGY   377(1) 275-283   2013年5月   [査読有り]
Nanos is a translational regulator required for the survival and maintenance of primordial germ cells during embryogenesis. Three nanos homologs are present in the genome of the sea urchin Strongylocentrotus puipuratus (Sp), and each nanos mRNA ac...
Hozumi, Akiko; Yoshida, Reiko; Horie, Takeo; Sakuma, Tetsushi; Yamamoto, Takashi; Sasakura, Yasunori
DEVELOPMENTAL BIOLOGY   375(1) 79-91   2013年3月   [査読有り]
Enhancers are flexible in terms of their location and orientation relative to the genes they regulate. However, little is known about whether the flexibility can be applied in every combination of enhancers and genes. Enhancer detection with trans...
Sakuma, Tetsushi; Hosoi, Sayaka; Woltjen, Knut; Suzuki, Ken-ichi; Kashiwagi, Keiko; Wada, Housei; Ochiai, Hiroshi; Miyamoto, Tatsuo; Kawai, Narudo; Sasakura, Yasunori et al.
GENES TO CELLS   18(4) 315-326   2013年4月   [査読有り]
Transcription activatorlike effector nucleases (TALENs) have recently arisen as effective tools for targeted genome engineering. Here, we report streamlined methods for the construction and evaluation of TALENs based on the Golden Gate TALEN and T...
High efficiency TALENs enable F0 functional analysis by targeted gene disruption in Xenopus laevis embryos
Suzuki KT, Isoyama Y, Kashiwagi K, Sakuma T, Ochiai H, Sakamoto N, Furuno N, Kashiwagi A, Yamamoto T
Biol Open      2013年3月
Efficient targeted mutagenesis in medaka using custom-designed transcription activator-like effector nucleases
Ansai S, Sakuma T, Yamamoto T, Ariga H, Uemura N, Takahashi R, Kinoshita M
Genetics      2013年3月
Quantitative assay for TALEN activity at endogenous genomic loci
Hisano Y, Ota S, Arakawa K, Muraki M, Kono N, Oshita K, Sakuma T, Tomita M, Yamamoto T, Okada Y, Kawahara A
Biol Open      2013年2月
Efficient gene targeting by TAL effector nucleases coinjected with exonucleases in zygotes
Mashimo T, Kaneko T, Sakuma T, Kobayashi J, Kunihiro Y, Voigt B, Yamamoto T, Serikawa T
Sci Rep      2013年
Efficient targeted mutagenesis of the chordate Ciona intestinalis genome with zinc-finger nucleases
Kawai N, Ochiai H, Sakuma T, Yamada L, Sawada H, Yamamoto T, Sasakura Y
Dev Growth Differ      2012年6月
Zinc-finger nuclease-mediated targeted insertion of reporter genes for quantitative imaging of gene expression in sea urchin embryos
Ochiai H, Sakamoto N, Fujita K, Nishikawa M, Suzuki K, Matsuura S, Miyamoto T, Sakuma T, Shibata T, Yamamoto T
Proc Natl Acad Sci USA      2012年7月
Non-transgenic genome modifications in a hemimetabolous insect using zinc-finger and TAL effector nucleases
Watanabe T, Ochiai H, Sakuma T, Horch HW, Hamaguchi N, Nakamura T, Bando T, Ohuchi H, Yamamoto T, Noji S, Mito T
Nat Commun      2012年
HpSumf1 is involved in the activation of sulfatases responsible for regulation of skeletogenesis during sea urchin development.
Sakuma T, Ohnishi K, Fujita K, Ochiai H, Sakamoto N, Yamamoto T
Dev Genes Evol      2011年8月
The third type III module of human fibronectin mediates cell adhesion and migration
J Biochem      2010年3月

書籍等出版物

 
1P-011 Platinum-Fungal TALENsを用いた麹菌におけるゲノム編集(遺伝子工学,一般講演)
水谷, 治;荒添, 貴之;利田, 賢次;林, 梨咲;大里, 修一;佐久間, 哲史;山本, 卓;桑田, 茂;山田, 修;Osamu, Mizutani;Takayuki, Arazoe;Kenji, Toshida;Risa, Hayashi;Shuichi, Ohsato;Tetsushi, Sakuma;Takashi, Yamamoto;Shigeru, Kuwata;Osamu, Yamada;酒総研;明治大院・農;広島大院・理;NRIB;Grad. Sch. Agric., Meiji Univ.;Grad. Sch. Sci., Hiroshima Univ.
2014年4月   
2P-098 TALEN を用いたゲノム編集によるジャガイモグリコアルカロイドの代謝改変(代謝工学,一般講演)
安本, 周平;關, 光;澤井, 学;大山, 清;梅基, 直行;佐久間, 哲史;山本, 卓;斉藤, 和季;村中, 俊哉;Shuhei, Yasumoto;Hikaru, Seki;Satoru, Sawai;Kiyoshi, Ohyama;Naoyuki, Umemoto;Tetsushi, Sakuma;Takashi, Yamamoto;Kazuki, Saito;Toshiya, Muranaka;阪大院・工・生命先端・生工;理研・環境資源科学研究セ;理研・環境資源科学研究セ:東工大院・理工・物質科学;キリン・基盤研;広島大院・理;Dept. Biotechnol., Div. Adv. Sci. Biotechnol., Grad. Sch. Eng., Osaka Univ.;CSRS, RIKEN;CSRS, RIKEN:Dept. Chem. Mater. Sci., Grad. Sch. Sci. Eng., Tokyo Tech;Kirin HD Co., Ltd.;Grad. Sch. Sci., Hiroshima Univ.
2017年4月   
P-004 TALENsを利用したメダカTLSポリメラーゼ遺伝子群変異体の網羅的作製(ポスターセッション,ゲノム変異の生成と抑制 : 分子メカニズムの理解からレギュラトリーサイエンスへ)
藤川, 芳宏;藤原(石川), 智子;佐久間, 哲史;山本, 卓;藤堂, 剛;Yoshihiro, Fujikawa;Tomoko, Fujiwara(Ishikawa);Tetsushi, Sakuma;Takashi, Yamamoto;Takeshi, Todo;大阪大学大学院医学系研究科;広島大学大学院理学研究科;Graduate School of Medicine, Osaka University;Graduate School of Science, Hiroshima University
2017年4月   
P-080 メダカにおけるTALENs利用した遺伝子破壊による損傷乗り越えDNA合成ポリメラーゼ変異体の作製(ポスターセッション,遺伝毒性と発がん : メカニズムを基軸とするリスク評価)
藤川, 芳宏;藤原(石川), 智子;佐久間, 哲史;山本, 卓;藤堂, 剛;Yoshihiro, Fuikawa;Tomoko, Fujiwara;Tetsushi, Sakukma;Takashi, Yamamoto;Takeshi, Todo;大阪大学大学院医学系研究科;広島大学大学院理学系研究科;Graduate School of Medicine, Osaka University;Graduate school of science, Hiroshima University
2017年4月   
P-074 メダカにおける人工ヌクレアーゼ(TALEN)を用いた損傷乗り越えポリメラーゼ変異体の作製(ポスターセッション)
藤川, 芳宏;藤原(石川), 智子;佐久間, 哲史;山本, 卓;藤堂, 剛;Yoshihiro, Fujikawa;Tomoko, (Ishikawa)Fujiwara;Tetsushi, Sakuma;Takashi, Yamamoto;Takeshi, Todo;大阪大学大学院医学系研究科;広島大学大学院理学研究科;Graduate school of medicine, Osaka University;Graduate school of science, Hiroshima University
2017年4月   

講演・口頭発表等

 
Updated summary of genome editing technology [招待有り]
11th International Symposium on Nanomedicine (ISNM2017)   2017年   
CRISPR-Cas9によるゲノム編集とヒト疾患解析・治療への応用 [招待有り]
第57回日本呼吸器学会学術講演会   2017年   
Recent advances in genome editing technology [招待有り]
The 23rd Annual Meeting of Japan Society of Gene and Cell Therapy   
培養細胞における遺伝子ノックイン技術の最新動向 [招待有り]
2017年度生命科学系学会合同年次大会(ConBio2017)   
Recent Development and Application of Genome Editing Tools and Methods [招待有り]
The 20th US-Japan Cellular and Gene Therapy Conference, CRISPR/Cas9 Gene Editing In Vivo   2017年   

競争的資金等の研究課題

 
プラチナTALE作製
日本医療研究開発機構: 革新的がん医療実用化研究事業
研究期間: 2017年4月 - 2018年3月    代表者: 佐久間 哲史
癌関連遺伝子の発現を多重制御するエピゲノム編集ベクターの開発と応用
日本医療研究開発機構: 革新的がん医療実用化研究事業
研究期間: 2017年4月 - 2018年3月    代表者: 佐久間 哲史
人工転写因子のデザインと機能評価
日本医療研究開発機構: 肝炎等克服実用化研究事業
研究期間: 2017年4月 - 2018年3月    代表者: 佐久間 哲史
癌関連遺伝子の発現を多重制御するエピゲノム編集ベクターの開発と応用
日本医療研究開発機構: 革新的がん医療実用化研究事業
研究期間: 2016年11月 - 2017年3月    代表者: 佐久間 哲史
エピゲノム変化誘導用プラチナTALEベクターシステムの確立
日本医療研究開発機構: 革新的がん医療実用化研究事業
研究期間: 2016年11月 - 2017年3月    代表者: 佐久間 哲史