2016年9月
Effects of overexpression of PKAc genes on expressions of lignin-modifying enzymes by Pleurotus ostreatus.
Bioscience, biotechnology, and biochemistry
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- 巻
- 80
- 号
- 9
- 開始ページ
- 1759
- 終了ページ
- 67
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1080/09168451.2016.1158630
- 出版者・発行元
- TAYLOR & FRANCIS LTD
We studied the role of genes encoding the cAMP-dependent protein kinase A catalytic subunit (PKAc) in the ligninolytic system in Pleurotus ostreatus. The wild-type P. ostreatus strain PC9 has two PKAc-encoding genes: PKAc1 and PKAc2 (protein ID 114122 and 85056). In the current study, PKAc1 and PKAc2 were fused with a β-tubulin promoter and introduced into strain PC9 to produce the overexpression strains PKAc1-97 and PKAc2-69. These strains showed significantly higher transcription levels of isozyme genes encoding lignin-modifying enzymes than strain PC9, but the specific gene expression patterns differed between the two recombinant strains. Both recombinants showed 2.05-2.10-fold faster degradation of beechwood lignin than strain PC9. These results indicate that PKAc plays an important role in inducing the wood degradation system in P. ostreatus.
- リンク情報
- ID情報
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- DOI : 10.1080/09168451.2016.1158630
- ISSN : 0916-8451
- eISSN : 1347-6947
- PubMed ID : 26979984
- Web of Science ID : WOS:000382588300010