2017年2月
Feline coronavirus replication is affected by both cyclophilin A and cyclophilin B
JOURNAL OF GENERAL VIROLOGY
- ,
- ,
- 巻
- 98
- 号
- 2
- 開始ページ
- 190
- 終了ページ
- 200
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1099/jgv.0.000663
- 出版者・発行元
- MICROBIOLOGY SOC
Feline coronavirus (FCoV) causes the fatal disease feline infectious peritonitis, which is currently incurable by drug treatment, and no effective vaccines are available. Cyclosporin A (CsA), a cyclophilin (Cyp) inhibitor, inhibits the replication of FCoV in vitro and in vivo as well as the replication of human and animal coronaviruses. However, the mechanism underlying the regulation of coronavirus replication by CsA is unknown. In this study, we analysed the role of Cyps in FCoV replication using knockdown and knockout cells specific to Cyps. Inhibition of CypA and CypB reduced FCoV replication, with replication in knockout cells being much less than that in knockdown cells. Furthermore, the proteins expressed by CypA and CypB harbouring mutations in their respective predicted peptidyl-prolyl cis-transisomerase active sites, which also alter the affinities between Cyps and CsA, inhibited FCoV replication. These findings indicate that the peptidyl-prolyl cistransisomerase active sites of Cyps might be required for FCoV replication.
- リンク情報
- ID情報
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- DOI : 10.1099/jgv.0.000663
- ISSN : 0022-1317
- eISSN : 1465-2099
- PubMed ID : 27902373
- Web of Science ID : WOS:000396115900009