論文

査読有り
2017年2月

Feline coronavirus replication is affected by both cyclophilin A and cyclophilin B

JOURNAL OF GENERAL VIROLOGY
  • Yoshikazu Tanaka
  • ,
  • Yuka Sato
  • ,
  • Takashi Sasaki

98
2
開始ページ
190
終了ページ
200
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1099/jgv.0.000663
出版者・発行元
MICROBIOLOGY SOC

Feline coronavirus (FCoV) causes the fatal disease feline infectious peritonitis, which is currently incurable by drug treatment, and no effective vaccines are available. Cyclosporin A (CsA), a cyclophilin (Cyp) inhibitor, inhibits the replication of FCoV in vitro and in vivo as well as the replication of human and animal coronaviruses. However, the mechanism underlying the regulation of coronavirus replication by CsA is unknown. In this study, we analysed the role of Cyps in FCoV replication using knockdown and knockout cells specific to Cyps. Inhibition of CypA and CypB reduced FCoV replication, with replication in knockout cells being much less than that in knockdown cells. Furthermore, the proteins expressed by CypA and CypB harbouring mutations in their respective predicted peptidyl-prolyl cis-transisomerase active sites, which also alter the affinities between Cyps and CsA, inhibited FCoV replication. These findings indicate that the peptidyl-prolyl cistransisomerase active sites of Cyps might be required for FCoV replication.

Web of Science ® 被引用回数 : 5

リンク情報
DOI
https://doi.org/10.1099/jgv.0.000663
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/27902373
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000396115900009&DestApp=WOS_CPL