2017年12月
Collagen-derived dipeptide prolyl-hydroxyproline promotes osteogenic differentiation through Foxg1
CELLULAR & MOLECULAR BIOLOGY LETTERS
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- 巻
- 22
- 号
- 27
- 開始ページ
- doi: 10.1186/s11658-017-0060-2
- 終了ページ
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1186/s11658-017-0060-2
- 出版者・発行元
- BIOMED CENTRAL LTD
Prolyl-hydroxyproline (Pro-Hyp) is one of the major constituents of collagen-derived dipeptides. We previously reported that Pro-Hyp promotes the differentiation of osteoblasts by increasing Runx2, osterix and Col1 alpha 1 mRNA expression levels. Here, to elucidate the mechanism of Pro-Hyp promotion of osteoblast differentiation, we focus on the involvement of Foxo1 in osteoblast differentiation via Runx2 regulation and the role of Foxg1 in Foxo1 regulation. The addition of Pro-Hyp had no effect on MC3T3-E1 cell proliferation in Foxo1- or Foxg1-knockdown cells. In Foxo1-knockdown cells, the addition of Pro-Hyp increased ALP activity, but in Foxg1-knockdown cells, it had no effect on ALP activity. An enhancing effect of Pro-Hyp on the Runx2 and osterix expression levels was observed in Foxo1-knockdown cells. However, no enhancing effect of Pro-Hyp on osteoblastic gene expression was observed when Foxg1 was knocked down. These results demonstrate that Pro-Hyp promotes osteoblastic MC3T3-E1 cell differentiation and upregulation of osteogenic genes via Foxg1 expression.
- リンク情報
- ID情報
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- DOI : 10.1186/s11658-017-0060-2
- ISSN : 1425-8153
- eISSN : 1689-1392
- Web of Science ID : WOS:000416829100002