Takayuki Tsukuba

Profile Information

Affiliation: Professor, Graduate School of Biomedical Sciences, Medical and Dental Sciences, Nagasaki University

J-GLOBAL ID: 200901050438500281
researchmap Member ID: 1000184231

1990年長崎大学歯学部卒業
1994年九州大学大学院歯学研究科修了
同年九州大学歯学部助手
2000年九州大学大学院歯学研究院助教授
2007年長崎大学大学院医歯薬学総合研究科教授

Research Interests

Research Areas

Research History

2007 - Present

Graduate School of Biomedical Sciences, Nagasaki University
2000 - 2007

Kyushu University
1994 - 2000

Kyushu University
1997 - 1999

The Pennsylvania State University

Education

- 1994

Kyushu University
- 1994

Graduate School, Division of Dental Research, Kyushu University
- 1990

School of Dentistry, Nagasaki University
- 1990

Faculty of Dentistry, Nagasaki University

Committee Memberships

Papers

110

Rab44 negatively regulates myoblast differentiation by controlling fusogenic protein transport and mTORC1 signaling.

Ayuko Tanimoto, Yu Yamaguchi, Tomoko Kadowaki, Eiko Sakai, Shun Oyakawa, Yusuke Ono, Noriaki Yoshida, Takayuki Tsukuba

Journal of Cellular Biochemistry, Aug 11, 2023 Last authorCorresponding author
Rab44 deficiency accelerates recovery from muscle damage by regulating mTORC1 signaling and transport of fusogenic regulators.

Shun Oyakawa, Yu Yamaguchi, Tomoko Kadowaki, Eiko Sakai, Mayuko Noguromi, Ayuko Tanimoto, Yusuke Ono, Hiroshi Murata, Takayuki Tsukuba

Journal of Cellular Physiology, Aug 10, 2023 Last authorCorresponding author
Abr, a Rho-regulating protein, modulates osteoclastogenesis by enhancing lamellipodia formation by interacting with poly(ADP-ribose) glycohydrolase.

Fatima Farhana, Eiko Sakai, Yu Koyanagi, Yu Yamaguchi, Mohammad Ibtehaz Alam, Kuniaki Okamoto, Takayuki Tsukuba

Molecular biology reports, Jul 28, 2023 Peer-reviewedLast authorCorresponding author
Rab44 Deficiency Induces Impaired Immune Responses to Nickel Allergy.

Mayuko Noguromi, Yu Yamaguchi, Keiko Sato, Shun Oyakawa, Kuniaki Okamoto, Hiroshi Murata, Takayuki Tsukuba, Tomoko Kadowaki

International journal of molecular sciences, 24(2), Jan 4, 2023 Peer-reviewed
Transposon mutagenesis and genome sequencing identify two novel, tandem genes involved in the colony spreading of Flavobacterium collinsii, isolated from an ayu fish, Plecoglossus altivelis.

Yoshio Kondo, Kenichi Ohara, Ryoji Fujii, Yudai Nakai, Chikara Sato, Mariko Naito, Takayuki Tsukuba, Tomoko Kadowaki, Keiko Sato

Frontiers in cellular and infection microbiology, 13 1095919-1095919, 2023 Peer-reviewed
HSP90 drives the Rab11a-mediated vesicular transport of the cell surface receptors in osteoclasts.

Manh Tien Tran, Yuka Okusha, Kaung Htike, Chiharu Sogawa, Takanori Eguchi, Tomoko Kadowaki, Eiko Sakai, Takayuki Tsukuba, Kuniaki Okamoto

Cell biochemistry and function, 40(8) 838-855, Sep 16, 2022 Peer-reviewed
Coronin1C Is a GDP-Specific Rab44 Effector That Controls Osteoclast Formation by Regulating Cell Motility in Macrophages.

Yu Yamaguchi, Tomoko Kadowaki, Nozomi Aibara, Kaname Ohyama, Kuniaki Okamoto, Eiko Sakai, Takayuki Tsukuba

International journal of molecular sciences, 23(12), Jun 14, 2022 Peer-reviewedLast authorCorresponding author
Rab34 plays a critical role as a bidirectional regulator of osteoclastogenesis

Yunxia Feng, Manh Tien Tran, Yanyin Lu, Kaung Htike, Yuka Okusha, Chiharu Sogawa, Takanori Eguchi, Tomoko Kadowaki, Eiko Sakai, Takayuki Tsukuba …

Cell Biochemistry and Function, Mar 14, 2022 Peer-reviewed
The Role of Cytokines Produced via the NLRP3 Inflammasome in Mouse Macrophages Stimulated with Dental Calculus in Osteoclastogenesis

Megumi Mae, Mohammad Ibtehaz Alam, Yasunori Yamashita, Yukio Ozaki, Kanako Higuchi, S. M. Ziauddin, Jorge Luis Montenegro Raudales, Eiko Sakai, Takayuki Tsukuba, Atsutoshi Yoshimura

International Journal of Molecular Sciences, 22(22) 12434-12434, Nov 18, 2021 Peer-reviewed
A novel role of HSP90 in regulating osteoclastogenesis by abrogating Rab11b-driven transport.

Manh Tien Tran, Yuka Okusha, Yunxia Feng, Chiharu Sogawa, Takanori Eguchi, Tomoko Kadowaki, Eiko Sakai, Takayuki Tsukuba, Kuniaki Okamoto

Biochimica et biophysica acta. Molecular cell research, 1868(10) 119096-119096, Sep, 2021 Peer-reviewed
Liquid-phase imaging of bone development and calcification by atmospheric scanning electron microscopy (ASEM): Application to immuno-labeling and rapid tissue observation of genetically modified mouse

Eiko Sakai, Mari Sato, Nassirhadjy Memtily, Takayuki Tsukuba, Chikara Sato

Microscopy and Microanalysis, 27(S1) 2288-2289, Aug, 2021
Large Rab GTPases: Novel Membrane Trafficking Regulators with a Calcium Sensor and Functional Domains.

Takayuki Tsukuba, Yu Yamaguchi, Tomoko Kadowaki

International journal of molecular sciences, 22(14), Jul 19, 2021 Lead authorCorresponding author
Rab44 isoforms similarly promote lysosomal exocytosis, but exhibit differential localization in mast cells.

Tomoko Kadowaki, Yu Yamaguchi, Kohei Ogawa, Mitsuko Tokuhisa, Kuniaki Okamoto, Takayuki Tsukuba

FEBS open bio, 11(4) 1165-1185, Apr, 2021 Peer-reviewedLast authorCorresponding author
Liquid-phase ASEM imaging of cellular and structural details in cartilage and bone formed during endochondral ossification: Keap1-deficient osteomalacia.

Eiko Sakai, Mari Sato, Nassirhadjy Memtily, Takayuki Tsukuba, Chikara Sato

Scientific reports, 11(1) 5722-5722, Mar 11, 2021 Peer-reviewed
The Inhibitory Role of Rab11b in Osteoclastogenesis through Triggering Lysosome-Induced Degradation of c-Fms and RANK Surface Receptors.

Manh Tien Tran, Yuka Okusha, Yunxia Feng, Masatoshi Morimatsu, Penggong Wei, Chiharu Sogawa, Takanori Eguchi, Tomoko Kadowaki, Eiko Sakai, Hirohiko Okamura …

International journal of molecular sciences, 21(24), Dec 8, 2020 Peer-reviewed
The large GTPase Rab44 regulates granule exocytosis in mast cells and IgE-mediated anaphylaxis.

Tomoko Kadowaki, Yu Yamaguchi, Mizuho A Kido, Takaya Abe, Kohei Ogawa, Mitsuko Tokuhisa, Weiqi Gao, Kuniaki Okamoto, Hiroshi Kiyonari, Takayuki Tsukuba

Cellular & molecular immunology, 17(12) 1287-1289, Dec, 2020 Peer-reviewedLast authorCorresponding author
Role of the EF-hand and coiled-coil domains of human Rab44 in localisation and organelle formation.

Kohei Ogawa, Tomoko Kadowaki, Mitsuko Tokuhisa, Yu Yamaguchi, Masahiro Umeda, Takayuki Tsukuba

Scientific reports, 10(1) 19149-19149, Nov 5, 2020 Peer-reviewedLast authorCorresponding author
Rab11A Functions as a Negative Regulator of Osteoclastogenesis through Dictating Lysosome-Induced Proteolysis of c-fms and RANK Surface Receptors.

Yuka Okusha, Manh Tien Tran, Mami Itagaki, Chiharu Sogawa, Takanori Eguchi, Tatsuo Okui, Tomoko Kadowaki, Eiko Sakai, Takayuki Tsukuba, Kuniaki Okamoto

Cells, 9(11), Oct 31, 2020 Peer-reviewed
Dimethyl fumarate prevents osteoclastogenesis by decreasing NFATc1 expression, inhibiting of erk and p38 MAPK phosphorylation, and suppressing of HMGB1 release.

Tsuyoshi Nishioku, Momomi Kawamoto, Ryuya Okizono, Eiko Sakai, Kuniaki Okamoto, Takayuki Tsukuba

Biochemical and biophysical research communications, 530(2) 455-461, Sep 17, 2020 Peer-reviewedLast author
Expression and localisation of Rab44 in immune-related cells change during cell differentiation and stimulation.

Mitsuko Tokuhisa, Tomoko Kadowaki, Kohei Ogawa, Yu Yamaguchi, Mizuho A Kido, Weiqi Gao, Masahiro Umeda, Takayuki Tsukuba

Scientific reports, 10(1) 10728-10728, Jul 1, 2020 Peer-reviewedLast authorCorresponding author

Misc.

高分子量Rabタンパク質による筋衛星細胞と筋形成過程に及ぼす影響

親川駿, 山口優, 門脇知子, 谷本あゆ子, 坂井詠子, 村田比呂司, 筑波隆幸

日本生化学会大会プログラム・講演要旨集, 95回 2P-168, Nov, 2022
高分子量Rabタンパク質が制御する筋芽細胞から筋管への分化機構

谷本あゆ子, 山口優, 門脇知子, 親川駿, 坂井詠子, 小野悠介, 吉田教明, 筑波隆幸

日本生化学会大会プログラム・講演要旨集, 95回 1P-171, Nov, 2022
Coronin 1CはGDP特異的Rab44エフェクターでありマクロファージの細胞運動を調節することで破骨細胞形成を制御する

山口優, 門脇知子, 相原希美, 大山要, 岡元邦彰, 坂井詠子, 筑波隆幸

日本生化学会大会プログラム・講演要旨集, 95回 1P-175, Nov, 2022
Rufy4はRUNドメインを介して破骨細胞による骨吸収を制御する

坂井詠子, Farhana Fatima, 小柳悠, 山口優, 筑波隆幸

日本生化学会大会プログラム・講演要旨集, 95回 2P-167, Nov, 2022
筋芽細胞分化でのRab44の制御機構

谷本あゆ子, 山口優, 筑波隆幸, 吉田教明

日本矯正歯科学会大会プログラム・抄録集, 81回 176-176, Oct, 2022
Rab44タンパク質による炎症応答制御機構(Regulatory mechanisms of inflammatory responses by Rab44 protein)

門脇知子, 山口優, 佐藤啓子, 筑波隆幸, 野黒美麻由子

Journal of Oral Biosciences Supplement, 2022 369-369, Sep, 2022
Keap1遺伝子欠損マウスにみられる軟骨内骨化の抑制 X線μCTおよび水中での大気圧走査電子顕微鏡による観察(Impaired endochondral ossification in Keap1-deficient mice: observation by X-ray μCT and atmospheric scanning electron microscopy in liquid)

坂井詠子, 佐藤主税, 佐藤真理, Fatima Farhana, 筑波隆幸

Journal of Oral Biosciences Supplement, 2021 320-320, Oct, 2021
歯科薬理学の新しい潮流リソソームとアレルギー疾患制御

門脇知子, 山口優, 筑波隆幸

Journal of Oral Biosciences Supplement, 2020 74-74, Sep, 2020
Lysosomal trafficking involved in allergic responses

門脇知子, 山口優, 筑波隆幸

Journal of Oral Biosciences Supplement (Web), 2020, 2020
コレステロール結晶はRAW-D細胞においてNLRP3インフラマソーム活性化とIL-1β産生を介して破骨細胞形成を促進する

樋口賀奈子, 山下恭徳, 尾崎幸生, 吉村篤利, 筑波隆幸, Montenegro Raudales Jorge Luis

特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 149回 58-58, Oct, 2018
破骨細胞分化を負に制御するRabタンパク質の同定

奥舎有加, 板垣まみ, 十川千春, 江口傑徳, 坂井詠子, 筑波隆幸, 岡元邦彰

Journal of Oral Biosciences Supplement, 2018 219-219, Sep, 2018
コレステロール結晶はRAW-D細胞の破骨細胞形成を促進する

Montenegro Raudales Jorge Luis, 山下恭徳, 尾崎幸生, 吉村篤利, 筑波隆幸

日本骨代謝学会学術集会プログラム抄録集, 36回 168-168, Jul, 2018
破骨細胞分化を負に制御するRabタンパク質の同定

奥舎有加, 板垣まみ, 十川千春, 江口傑徳, 江口傑徳, 坂井詠子, 筑波隆幸, 岡元邦彰

Journal of Oral Biosciences Supplement (Web), 2018, 2018
Rab44タンパク質の細胞内局在とその制御に関する解析

小川晃平, 門脇知子, 徳久美都子, 山口優, 梅田正博, 筑波隆幸

日本分子生物学会年会プログラム・要旨集(Web), 41st, 2018 Last authorCorresponding author
マウスにおけるRab44の組織分布および発現変動の解析

徳久美都子, 門脇知子, 小川晃平, 山口優, 梅田正博, 筑波隆幸

日本分子生物学会年会プログラム・要旨集(Web), 41st, 2018 Last authorCorresponding author
Keap1遺伝子欠損はNrf2の活性化を介して破骨細胞分化を抑制する

坂井詠子, 福間裕, 西下一久, 岡元邦彰, 筑波隆幸

生命科学系学会合同年次大会, 2017年度 [1P-0588], Dec, 2017
扁平上皮癌細胞の増殖能・浸潤能・転移能における転写因子TFEBの役割

坂元裕, 山下健太郎, 岡元邦彰, 門脇知子, 梅田正博, 筑波隆幸

生命科学系学会合同年次大会, 2017年度 [1P-1012], Dec, 2017
コレステロール結晶はNLRP3インフラマソームを活性化してマウスマクロファージにおけるIL-1β産生と破骨細胞形成を促進する

樋口賀奈子, 吉村篤利, 白石千秋, 原宜興, 筑波隆幸

特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 147回 118-118, Oct, 2017
核内タンパク質HMGM1 ヘムオキシゲナーゼ1の発現抑制はカスパーゼ3の活性化、HMGB1の細胞外遊離と破骨細胞分化に必要である

坂井詠子, 岡元邦彰, 筑波隆幸

Journal of Oral Biosciences Supplement, 2017 124-124, Sep, 2017
Keap1遺伝子欠損はIRF-8とMafBの発現上昇を介して破骨細胞分化を抑制する

坂井詠子, 城戸瑞穂, 福間裕, 西下一久, 岡元邦彰, 筑波隆幸

Journal of Oral Biosciences Supplement, 2017 241-241, Sep, 2017

Teaching Experience

薬理学、歯科薬理学 (長崎大学歯学部、九州大学歯学部)

Professional Memberships

Research Projects

Development of multifunctional soft denture liners with self-cleaning function and drug delivery function for sustained release of physiologically active substances

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Japan Society for the Promotion of Science, Apr, 2023 - Mar, 2026
変異型Rufy4を発現する破骨細胞の細胞外小胞を介した骨吸収調節機構の解明と応用

科学研究費助成事業, 基盤研究(C), 日本学術振興会, Apr, 2023 - Mar, 2026

坂井詠子, 筑波隆幸, 山口優, 佛坂斉祉, 近藤好夫, 永野健一
Functional analysis of a novel membrane transport factor in metabolic bone diseases

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Japan Society for the Promotion of Science, Apr, 2023 - Mar, 2026
歯周病発症における新規小胞輸送因子の分子機構の解析

科学研究費助成事業基盤研究(C), 基盤研究(C), 日本学術振興会, Apr, 2021 - Mar, 2024

近藤好夫, 筑波隆幸, 門脇知子, 鵜飼孝, 佐藤啓子, 藤原卓
Multimodal imaging analysis of oral cancer cells focused on lysosomes

Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Grant-in-Aid for Scientific Research (C), Japan Society for the Promotion of Science, Apr, 2021 - Mar, 2024
Development of multifunctional viscoelastic soft denture liners with controlled release ability of biologically active agent and photocatalyst function using nanocomposites

Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B), Grant-in-Aid for Scientific Research (B), Japan Society for the Promotion of Science, Apr, 2020 - Mar, 2023
Functional and pathological analyses of inflammatory bone destruction disease by a novel Rab protein

Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B), Grant-in-Aid for Scientific Research (B), Japan Society for the Promotion of Science, Apr, 2020 - Mar, 2023
酸化ストレスと骨吸収に関連した新規オートファジー制御因子の機能解明

科学研究費助成事業基盤研究(C), 基盤研究(C), 日本学術振興会, Apr, 2019 - Mar, 2022

坂井詠子, 筑波隆幸, 山口優
破骨細胞における新規輸送因子の同定と機能解明

科学研究費助成事業基盤研究(C), 基盤研究(C), 日本学術振興会, Apr, 2019 - Mar, 2022

福間裕, 筑波隆幸, 坂井詠子
Function of a novel Rab protein in neutrophils and its role in periodontal disease

Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Grant-in-Aid for Scientific Research (C), Japan Society for the Promotion of Science, Apr, 2019 - Mar, 2022

Yukitake Hideharu
Regulatory mechanism of mast cell degranulation by the novel Rab protein

Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Grant-in-Aid for Scientific Research (C), Japan Society for the Promotion of Science, Apr, 2018 - Mar, 2021

Kadowaki Tomoko
光触媒機能と治癒促進機能をもつダイナミック印象応用型光重合軟質リライン材の開発

科学研究費助成事業基盤研究(B), 基盤研究(B), 日本学術振興会, Apr, 2017 - Mar, 2020

村田比呂司, 小椎尾謙, 筑波隆幸, 稲光宏之
Discovery of a novel gene regulating membrane trafficking of immune cells and elucidation of its pathology

Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B), Grant-in-Aid for Scientific Research (B), Japan Society for the Promotion of Science, Apr, 2017 - Mar, 2020

TSUKUBA Takayuki
Molecular mechanism of bone destruction in rheumatoid arthritis based on CD147

Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Grant-in-Aid for Scientific Research (C), Japan Society for the Promotion of Science, Apr, 2016 - Mar, 2019

NISHIOKU TSUYOSHI
Elucidation of Keap1/Nrf2-mediated control system of bone metabolism and application to in silico drug discovery

Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Grant-in-Aid for Scientific Research (C), Japan Society for the Promotion of Science, Apr, 2016 - Mar, 2019

SAKAI Eiko
Development of Supplement having anti-cancer action based on Flavonoid

Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Grant-in-Aid for Scientific Research (C), Japan Society for the Promotion of Science, Apr, 2016 - Mar, 2019

OKAMOTO Kuniaki
破骨細胞におけるリソソーム分泌機構の制御因子の同定と分子メカニズムの解析

科学研究費助成事業基盤研究(C), 基盤研究(C), 日本学術振興会, Apr, 2016 - Mar, 2018

西下一久, 筑波隆幸, 岡元邦彰, 坂井詠子
Analyses using bioimaging of invasion and colonization mechanisms by periodontal bacteria in the liver

Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research, Grant-in-Aid for Challenging Exploratory Research, Japan Society for the Promotion of Science, Apr, 2016 - Mar, 2018

TSUKUBA Takayuki
Elucidation of chronic inflammation exacerbation mechanism due to breakdown of endo-lysosomal proteolysis

Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Grant-in-Aid for Scientific Research (C), Japan Society for the Promotion of Science, Apr, 2015 - Mar, 2018

KADOWAKI Tomoko
Examination of new medicine for bone metabolism by investigation of potential natural drugs in China

Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B), Grant-in-Aid for Scientific Research (B), Japan Society for the Promotion of Science, Apr, 2015 - Mar, 2018

TSUKUBA Takayuki

Profile Information

Research Interests

Research Areas

Research History

Education

Committee Memberships

Papers

Misc.

Teaching Experience

Professional Memberships

Research Projects

Menu

Coauthors